ArticleScientific reports2018
Rapid coupling between gravitational forces and the transcriptome in human myelomonocytic U937 cells.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 38 citations in OpenAlex.
- Gravity as a Boundary Condition for the Evolution of Three-Dimensional Multicellularity.Life (Basel, Switzerland) · 2026Article
- Astroimmunology: the effects of spaceflight and its associated stressors on the immune system.Nature reviews. Immunology · 2026Review
- Exploring the Impact of Microgravity on Gene Expression: Dysregulated Pathways and Candidate Repurposed Drugs.International journal of molecular sciences · 2025Article
- NASA GeneLab derived microarray studies of Mus musculus and Homo sapiens organisms in altered gravitational conditions.NPJ microgravity · 2024Review
- Article
- Omics Studies of Tumor Cells under Microgravity Conditions.International journal of molecular sciences · 2024Review
- Increased H3K9me3 and F-Actin Reorganization in the Rapid Adaptive Response to Hypergravity in Human T Lymphocytes.International journal of molecular sciences · 2023Article
- The Impact of Microgravity on Immunological States.ImmunoHorizons · 2023Review
- Transcriptional Response in Human Jurkat T Lymphocytes to a near Physiological Hypergravity Environment and to One Common in Routine Cell Culture Protocols.International journal of molecular sciences · 2023Article
- Rapid Downregulation of H3K4me3 Binding to Immunoregulatory Genes in Altered Gravity in Primary Human M1 Macrophages.International journal of molecular sciences · 2022Article
- Single Cell in a Gravity Field.Life (Basel, Switzerland) · 2022Review
- Article
- Signatures of muscle disuse in spaceflight and bed rest revealed by single muscle fiber proteomics.PNAS nexus · 2022Article
- Post-Transcriptional Dynamics is Involved in Rapid Adaptation to Hypergravity in Jurkat T Cells.Frontiers in cell and developmental biology · 2022Article
- Genomic Changes Driven by Radiation-Induced DNA Damage and Microgravity in Human Cells.International journal of molecular sciences · 2021Review
- Gravitational Force-Induced 3D Chromosomal Conformational Changes Are Associated with Rapid Transcriptional Response in Human T Cells.International journal of molecular sciences · 2021Article
- Rapid Transient Transcriptional Adaptation to Hypergravity in Jurkat T Cells Revealed by Comparative Analysis of Microarray and RNA-Seq Data.International journal of molecular sciences · 2021Article
- Metabolic Dynamics in Short- and Long-Term Microgravity in Human Primary Macrophages.International journal of molecular sciences · 2021Article
- Macrophages in microgravity: the impact of space on immune cells.NPJ microgravity · 2021Review
- Modifications of Plasma Membrane Organization in Cancer Cells for Targeted Therapy.Molecules (Basel, Switzerland) · 2021Review
Corrections and comments
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Authors and funding
14 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gravitational force has been constant throughout Earth's evolutionary history. Since the cell nucleus is subjected to permanent forces induced by Earth's gravity, we addressed the question, if gene expression homeostasis is constantly shaped by the gravitational force on Earth. We therefore investigated the transcriptome in force-free conditions of microgravity, determined the time frame of initial gravitational force-transduction to the transcriptome and assessed the role of cation channels. We combined a parabolic flight experiment campaign with a suborbital ballistic rocket experiment employing the human myelomonocytic cell line U937 and analyzed the whole gene transcription by microarray, using rigorous controls for exclusion of effects not related to gravitational force and cross-validation through two fully independent research campaigns. Experiments with the wide range ion channel inhibitor SKF-96365 in combination with whole transcriptome analysis were conducted to study the functional role of ion channels in the transduction of gravitational forces at an integrative level. We detected profound alterations in the transcriptome already after 20 s of microgravity or hypergravity. In microgravity, 99.43% of all initially altered transcripts adapted after 5 min. In hypergravity, 98.93% of all initially altered transcripts adapted after 75 s. Only 2.4% of all microgravity-regulated transcripts were sensitive to the cation channel inhibitor SKF-96365. Inter-platform comparison of differentially regulated transcripts revealed 57 annotated gravity-sensitive transcripts. We assume that gravitational forces are rapidly and constantly transduced into the nucleus as omnipresent condition for nuclear and chromatin structure as well as homeostasis of gene expression.
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