ArticleMicrobes and environments2018
Development of a Quantitative PCR Assay for Arcobacter spp. and its Application to Environmental Water Samples.
Article in Microbes and environments, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- Diverse Members of the PhylumInternational journal of molecular sciences · 2025Article
- Microbial and Chemical Water Quality Assessments Across the Rural and Urban Areas of Nepal: A Scoping Review.International journal of environmental research and public health · 2025Article
- Bacterivorous CiliateAntibiotics (Basel, Switzerland) · 2025Article
- Comparison of the Diagnostic Accuracy of Three Real-Time PCR Assays for the Detection ofMicroorganisms · 2023Article
- The Prevalence ofPathogens (Basel, Switzerland) · 2022Review
- The Persistence of Bacterial Pathogens in Surface Water and Its Impact on Global Food Safety.Pathogens (Basel, Switzerland) · 2021Review
- Real-time quantitative PCR assay development and application for assessment of agricultural surface water and various fecal matter for prevalence of Aliarcobacter faecis and Aliarcobacter lanthieri.BMC microbiology · 2020Article
- Reduction ofPathogens (Basel, Switzerland) · 2019Article
- Identification of 16S rRNA and Virulence-Associated Genes ofPathogens (Basel, Switzerland) · 2019Article
- The unexpected habitat in sewer pipes for the propagation of microbial communities and their imprint on urban waters.Current opinion in biotechnology · 2019Review
- Ecology of Pathogens and Antibiotic-resistant Bacteria in Environments: Challenges and Opportunities.Microbes and environmentsArticle
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Arcobacter spp. are emerging pathogens associated with gastroenteritis in humans. The objective of this study was to develop a highly sensitive and broadly reactive quantitative PCR (qPCR) assay for Arcobacter spp. and to apply the developed assay to different water sources in the Kathmandu Valley, Nepal. Fifteen samples to be analyzed by next-generation sequencing were collected from 13 shallow dug wells, a deep tube well, and a river in the Kathmandu Valley in August 2015. Among the 86 potential pathogenic bacterial genera identified, Acinetobacter, Pseudomonas, Flavobacterium, and Arcobacter were detected with relatively high abundance in 15, 14, 12, and 8 samples, respectively. A primer pair was designed with maximal nucleotide homologies among Arcobacter spp. by comparing the sequences of 16S rRNA genes. These primers were highly specific to most of the known species of Arcobacter and quantified between 1.0×10
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.