ArticleJCI insight2018
Mouse model of Gram-negative prosthetic joint infection reveals therapeutic targets.
Article in JCI insight, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 34 citations in OpenAlex.
- Current therapeutic interventions combating biofilm-related infections in orthopaedics : a systematic review of in vivo animal studies.Bone & joint research · 2022Pooled it
- Single-dose dual-indication nanoparticle vaccine co-displayingMaterials today. Bio · 2026Article
- In vivo bacteria-targeted imaging with vancomycin-based positron emission tomography and optical tracers in an orthopaedic trauma implant infection model.European journal of nuclear medicine and molecular imaging · 2026Article
- Effect of sonication on staphylococcal and gram-negative biofilms relevant to prosthetic joint infections: an in vitro study.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Does fluid irrigation in arthroscopic shoulder biopsy affect sensitivity ofJSES international · 2025Article
- MappingMicroorganisms · 2024Article
- Peptide-mimetic treatment of Pseudomonas aeruginosa in a mouse model of respiratory infection.Communications biology · 2024Article
- Neutrophil-intrinsic TNF receptor signaling orchestrates host defense againstScience advances · 2023Article
- Development of an anti-Frontiers in cellular and infection microbiology · 2023Article
- Polymyxin B in Combination with Glycerol Monolaurate Exerts Synergistic Killing against Gram-Negative Pathogens.Pathogens (Basel, Switzerland) · 2022Article
- Staphylococcus aureus and Acinetobacter baumannii Inhibit Osseointegration of Orthopedic Implants.Infection and immunity · 2022Article
- CCR2 contributes to host defense against Staphylococcus aureus orthopedic implant-associated infections in mice.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2022Article
- Development of an innovativeFrontiers in medicine · 2022Article
- Recent Advances in the Evaluation of Antimicrobial Materials for Resolution of Orthopedic Implant-Associated InfectionsACS infectious diseases · 2021Review
- An Organ System-Based Synopsis ofVirulence · 2021Review
- Predilection for developing a hematogenous orthopaedic implant-associated infection in older versus younger mice.Journal of orthopaedic surgery and research · 2021Article
- Therapeutic assessment of N-formyl-methionyl-leucyl-phenylalanine (fMLP) in reducing periprosthetic joint infection.European cells & materials · 2021Article
- Pan-caspase inhibition as a potential host-directed immunotherapy against MRSA and other bacterial skin infections.Science translational medicine · 2021Article
- A trilogy antimicrobial strategy for multiple infections of orthopedic implants throughout their life cycle.Bioactive materials · 2021Article
- Functionalization of Synthetic Bone Substitutes.International journal of molecular sciences · 2021Review
Corrections and comments
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Authors and funding
18 authors at 2 institutions in 1 country.
Funding
Abstract
Bacterial biofilm infections of implantable medical devices decrease the effectiveness of antibiotics, creating difficult-to-treat chronic infections. Prosthetic joint infections (PJI) are particularly problematic because they require prolonged antibiotic courses and reoperations to remove and replace the infected prostheses. Current models to study PJI focus on Gram-positive bacteria, but Gram-negative PJI (GN-PJI) are increasingly common and are often more difficult to treat, with worse clinical outcomes. Herein, we sought to develop a mouse model of GN-PJI to investigate the pathogenesis of these infections and identify potential therapeutic targets. An orthopedic-grade titanium implant was surgically placed in the femurs of mice, followed by infection of the knee joint with Pseudomonas aeruginosa or Escherichia coli. We found that in vitro biofilm-producing activity was associated with the development of an in vivo orthopedic implant infection characterized by bacterial infection of the bone/joint tissue, biofilm formation on the implants, reactive bone changes, and inflammatory immune cell infiltrates. In addition, a bispecific antibody targeting P. aeruginosa virulence factors (PcrV and Psl exopolysaccharide) reduced the bacterial burden in vivo. Taken together, our findings provide a preclinical model of GN-PJI and suggest the therapeutic potential of targeting biofilm-associated antigens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.