ArticleDevelopment (Cambridge, England)2018
Targeted DamID reveals differential binding of mammalian pluripotency factors.
Article in Development (Cambridge, England), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 53 citations in OpenAlex.
- damidBind: an R/bioconductor package for differential DamID analysis and data exploration.Bioinformatics (Oxford, England) · 2026Article
- PLAMseq enables the proteo-genomic characterization of chromatin-associated proteins and protein interactions in a single workflow.Science advances · 2025Article
- Mapping SET1B chromatin interactions with DamID using DamMapper, a comprehensive Snakemake workflow.BMC genomics · 2025Article
- NKX2-5 congenital heart disease mutations show diverse loss and gain of epigenomic, biochemical and chromatin search functions underpinning pathogenicity.bioRxiv : the preprint server for biology · 2025Article
- Loss of cytoplasmic actin filaments raises nuclear actin levels to drive INO80C-dependent chromosome fragmentation.Nature communications · 2024Article
- Article
- Epigenetic resetting in the human germ line entails histone modification remodeling.Science advances · 2023Article
- Mouse primordial germ-cell-like cells lack piRNAs.Developmental cell · 2022Article
- SOX9 maintains human foetal lung tip progenitor state by enhancing WNT and RTK signalling.The EMBO journal · 2022Article
- Single-cell profiling of transcriptome and histone modifications with EpiDamID.Molecular cell · 2022Article
- NanoDam identifies Homeobrain (ARX) and Scarecrow (NKX2.1) as conserved temporal factors in the Drosophila central brain and visual system.Developmental cell · 2022Article
- Understanding the impact of ZBTB18 missense variation on transcription factor function in neurodevelopment and disease.Journal of neurochemistry · 2022Review
- Reduced chromatin accessibility correlates with resistance to Notch activation.Nature communications · 2022Article
- Targeted DamID inScience advances · 2022Article
- Profiling Protein-DNA Interactions Cell-Type-Specifically with Targeted DamID.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Cardiovascular Progerin Suppression and Lamin A Restoration Rescue Hutchinson-Gilford Progeria Syndrome.Circulation · 2021Article
- Article
- Brain on food: The neuroepigenetics of nutrition.Neurochemistry international · 2021Review
- FlyORF-TaDa allows rapid generation of new lines for in vivo cell-type-specific profiling of protein-DNA interactions in Drosophila melanogaster.G3 (Bethesda, Md.) · 2021Article
- Tissue-specific DamID protocol using nanopore sequencing.Journal of biological methods · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
The precise control of gene expression by transcription factor networks is crucial to organismal development. The predominant approach for mapping transcription factor-chromatin interactions has been chromatin immunoprecipitation (ChIP). However, ChIP requires a large number of homogeneous cells and antisera with high specificity. A second approach, DamID, has the drawback that high levels of Dam methylase are toxic. Here, we modify our targeted DamID approach (TaDa) to enable cell type-specific expression in mammalian systems, generating an inducible system (mammalian TaDa or MaTaDa) to identify genome-wide protein/DNA interactions in 100 to 1000 times fewer cells than ChIP-based approaches. We mapped the binding sites of two key pluripotency factors, OCT4 and PRDM14, in mouse embryonic stem cells, epiblast-like cells and primordial germ cell-like cells (PGCLCs). PGCLCs are an important system for elucidating primordial germ cell development in mice. We monitored PRDM14 binding during the specification of PGCLCs, identifying direct targets of PRDM14 that are key to understanding its crucial role in PGCLC development. We show that MaTaDa is a sensitive and accurate method for assessing cell type-specific transcription factor binding in limited numbers of cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.