ArticleBMC cancer2018
Increased insulin-like growth factor 1 production by polyploid adipose stem cells promotes growth of breast cancer cells.
Article in BMC cancer, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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13 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.
- The effects of physical activity on physiological markers in breast cancer survivors: A meta-analysis.Medicine · 2020Pooled it
- The adenovirus E4orf1 protein initiates a feedback loop involving insulin and growth factor receptors, AKT, and NF-κB, leading to abnormal DNA content in infected cells.PLoS pathogens · 2025Article
- Polyploidy Promotes Hypertranscription, Apoptosis Resistance, and Ciliogenesis in Cancer Cells and Mesenchymal Stem Cells of Various Origins: Comparative Transcriptome In Silico Study.International journal of molecular sciences · 2024Article
- Functional consequences of somatic polyploidy in development.Development (Cambridge, England) · 2024Review
- Serum insulin-like growth factor-1 as a potential prognostic biomarker for heart failure with reduced ejection fraction: a meta-analysis.Frontiers in cardiovascular medicine · 2024Article
- Stem cells implanted with nanofibrous mats for injured endometrial regeneration and immune-microenvironment remodeling.Materials today. Bio · 2023Article
- Lipofilling in Breast Oncological Surgery: A Safe Opportunity or Risk for Cancer Recurrence?International journal of molecular sciences · 2021Review
- Lung proliferative lesion-promoting effects of left pulmonary ligation in A/J female mice.Pathology international · 2020Article
- Role of Bcl-2 on drug resistance in breast cancer polyploidy-induced spindle poisons.Oncology letters · 2020Article
- Polyploid Adipose Stem Cells Shift the Balance of IGF1/IGFBP2 to Promote the Growth of Breast Cancer.Frontiers in oncology · 2020Article
- Increase in IGF-1 Expression in the Injured Infraorbital Nerve and Possible Implications for Orofacial Neuropathic Pain.International journal of molecular sciences · 2019Article
- Canine Bone Marrow-derived Mesenchymal Stem Cells: Genomics, Proteomics and Functional Analyses of Paracrine Factors.Molecular & cellular proteomics : MCP · 2019Article
- The clinical significance of CHEK1 in breast cancer: a high-throughput data analysis and immunohistochemical study.International journal of clinical and experimental pathology · 2019Article
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10 authors at 1 institution in 1 country.
Funding
Abstract
backgroundAdipose-tissue stem cells (ASCs) are subject of intensive research since their successful use in regenerative therapy. The drawback of ASCs is that they may serve as stroma for cancer cells and assist tumor progression. It is disquieting that ASCs frequently undergo genetic and epigenetic changes during their in vitro propagation. In this study, we describe the polyploidization of murine ASCs and the accompanying phenotypical, gene expressional and functional changes under long term culturing.
methodsASCs were isolated from visceral fat of C57BL/6 J mice, and cultured in vitro for prolonged time. The phenotypical changes were followed by microscopy and flow cytometry. Gene expressional changes were determined by differential transcriptome analysis and changes in protein expression were shown by Western blotting. The tumor growth promoting effect of ASCs was examined by co-culturing them with 4 T1 murine breast cancer cells.
resultsAfter five passages, the proliferation of ASCs decreases and cells enter a senescence-like state, from which a proportion of cells escape by polyploidization. The resulting ASC line is susceptible to adipogenic, osteogenic and chondrogenic differentiation, and expresses the stem cell markers CD29 and Sca-1 on an upregulated level. Differential transcriptome analysis of ASCs with normal and polyploid karyotype shows altered expression of genes that are involved in regulation of cancer, cellular growth and proliferation. We verified the increased expression of Klf4 and loss of Nestin on protein level. We found that elevated production of insulin-like growth factor 1 by polyploid ASCs rendered them more potent in tumor growth promotion in vitro.
conclusionsOur model indicates how ASCs with altered genetic background may support tumor progression.
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