ArticleMedical science monitor : international medical journal of experimental and clinical research2018
Distinct Anti-Fibrotic Effects of Exosomes Derived from Endothelial Colony-Forming Cells Cultured Under Normoxia and Hypoxia.
Article in Medical science monitor : international medical journal of experimental and clinical research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Ubiquitin proteasome system in cardiac fibrosis.Journal of advanced research · 2025Review
- Recent advances in endothelial colony-forming cells: from the transcriptomic perspective.Journal of translational medicine · 2024Review
- The Roles of Exosome-Derived microRNAs in Cardiac Fibrosis.Molecules (Basel, Switzerland) · 2024Review
- Boosting extracellular vesicle secretion.Biotechnology advances · 2022Review
- Tumor endothelial cell-derived extracellular vesicles contribute to tumor microenvironment remodeling.Cell communication and signaling : CCS · 2022Review
- Hypoxia Induced Changes of Exosome Cargo and Subsequent Biological Effects.Frontiers in immunology · 2022Review
- Diagnostic and Therapeutic Properties of Exosomes in Cardiac Fibrosis.Frontiers in cell and developmental biology · 2022Review
- Review
- Review
- The Role of HECT-Type E3 Ligase in the Development of Cardiac Disease.International journal of molecular sciences · 2021Review
- Latest Advances in Endothelial Progenitor Cell-Derived Extracellular Vesicles Translation to the Clinic.Frontiers in cardiovascular medicine · 2021Review
- Cardioprotective Roles of Endothelial Progenitor Cell-Derived Exosomes.Frontiers in cardiovascular medicine · 2021Review
- Cardiac Fibrosis: Cellular Effectors, Molecular Pathways, and Exosomal Roles.Frontiers in cardiovascular medicine · 2021Review
- Hypoxia and extracellular vesicles: A review on methods, vesicular cargo and functions.Journal of extracellular vesicles · 2020Review
- Non-coding RNAs in Cardiac Intercellular Communication.Frontiers in physiology · 2020Review
- E3 Ubiquitin ligase NEDD4 family‑regulatory network in cardiovascular disease.International journal of biological sciences · 2020Review
- Advances in Exosomes Derived from Different Cell Sources and Cardiovascular Diseases.BioMed research international · 2020Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND The therapeutic potential of endothelial colony-forming cells (ECFCs) may be impaired in an ischemic environment. Direct injection of ECFCs is not an effective method of rescuing the ischemic heart, but exosomes derived from these cells may be a promising therapeutic tool. However, exosomes produced under normoxia and hypoxia may not be identical. Therefore, the purpose of this study was to investigate alterations in the anti-fibrotic effects of hypoxia-treated ECFC-derived exosomes and the underlying mechanism involved. MATERIAL AND METHODS ECFCs were isolated from peripheral blood and exosomes were collected from ECFCs treated with normoxia (nor-exo) or hypoxia (hyp-exo). Effects of exosomes on cardiac fibroblast activation were evaluated in vitro. MicroRNAs (miRNAs) inside the exosomes were extracted and compared using next-generation RNA sequencing. Predicted target mRNAs of miR-10b-5p were validated using a dual-luciferase reporter gene assay method. RESULTS Nor-exo significantly ameliorated cardiac fibroblast activation in vitro. These effects were attenuated in the hyp-exo-treated group. miR-10b-5p was enriched in nor-exo but not in hyp-exo. Dual-luciferase reporter gene assay found that both SMAD-specific E3 ubiquitin protein ligase 1 (Smurf1) and histone deacetylase 4 (HDAC4) mRNAs were inhibited by miR-10b-5p. The expression of neutral sphingomyelinase 2 (N-SMase2) was decreased in hypoxia ECFCs, and this result was consistent with the changes in miR-10b-5p in hyp-exo. CONCLUSIONS Due to a reduction of miR-10b-5p, which targets the fibrotic genes Smurf1 and HDAC4, the anti-fibrotic effects of hyp-exo were abolished.
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