Sequential Phosphopeptide Enrichment for Phosphoproteome Analysis of Filamentous Fungi: A Test Case Using Magnaporthe oryzae.

Yeonyee Oh, William L Franck, Ralph A Dean

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Yeonyee OhFungal Genomics Lab, Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, USA.

William L FranckFungal Genomics Lab, Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, USA.

Ralph A DeanFungal Genomics Lab, Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, USA. radean2@ncsu.edu.

Funding

Tri-Institutional Molecular Mycology and Pathogenesis Training ProgramT32AI052080 · NIAID · DUKE UNIVERSITY · PI ANDREW ALSPAUGH, JOSEPH HEITMAN · 2003 to 2026

$8.7M

Genetics Training Grant-Year 26T32GM007754 · NIGMS · DUKE UNIVERSITY · PI HAASE, STEVEN B · 1985 to 2019

$8.3M

NIAID NIH HHS T32 AI052080NIGMS NIH HHS T32 GM007754

6 · The paper itself

Abstract

A number of challenges have to be overcome to identify a complete complement of phosphorylated proteins, the phosphoproteome, from cells and tissues. Phosphorylated proteins are typically of low abundance and moreover, the proportion of phosphorylated sites on a given protein is generally low. The challenge is further compounded when the tissue from which protein can be recovered is limited. Global phosphoproteomics primarily relies on efficient enrichment methods for phosphopeptides involving affinity binding coupled with analysis by fast high-resolution mass spectrometry (MS) and subsequent identification using various software packages. Here, we describe an effective protocol for phosphopeptide enrichment using an Iron-IMAC resin in combination with titanium dioxide (TiO

Indexed as

ProteomeProteomicsChromatography, AffinityChromatography, LiquidComputational BiologyData AnalysisFungal ProteinsHumansMagnaporthePhosphopeptidesPhosphoproteinsTandem Mass SpectrometryTitaniumFungal ProteinsPhosphopeptidesPhosphoproteinsProteomeTitaniumtitanium dioxideFungusIron-IMACMagnaporthe oryzaeMass spectrometryPhosphopeptide enrichmentTiO2

Identifiers

PMID30182230

DOI10.1007/978-1-4939-8724-5_7

PMCPMC6502229

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.