ArticlePlant molecular biology2018
The ethylene response factor MdERF1B regulates anthocyanin and proanthocyanidin biosynthesis in apple.
Article in Plant molecular biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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82 citing papers in PubMed, 204 citations in OpenAlex.
- Plant Flavonoids: Biosynthesis, Regulation, and Roles in Biotic and Abiotic Stresses.Plant, cell & environment · 2026Review
- Ethylene-responsive CmERF3-CmMYB4 cascade suppresses anthocyanin biosynthesis inHorticulture research · 2026Article
- Transcriptomic and hormonic analyses reveal the effects of xenia on anthocyanin biosynthesis in blueberry.Food chemistry. Molecular sciences · 2026Article
- Integrated Transcriptomics and Metabolomics with Machine Learning Identify Flavonoids as Key Effectors in Wheat Root Thermotolerance.Plants (Basel, Switzerland) · 2026Article
- Genome Assembly Revealed MdZAT5 Coordinates Anthocyanin Biosynthesis in Apple Fruit Peel and Flesh by Interacting With MdHY5.Plant biotechnology journal · 2026Article
- Integrated transcriptomic and metabolomic profiling reveals the flower color formation mechanism of alfalfa different purple flowers.Frontiers in plant science · 2026Article
- Identification and analysis of the AP2/ERF gene family inFrontiers in plant science · 2026Article
- AP2/ERF transcription factors regulate the biosynthesis of terpenoids, phenolics, and alkaloids in plants.Horticulture research · 2026Article
- Metabolic profiling of red-fleshed apple and functional characterization of MdERF072 in anthocyanin biosynthesis.BMC plant biology · 2025Article
- Molecular mechanism of ethylene signaling response gene VcERF061 to promote anthocyanin synthesis in blueberry (Vaccinium corymbosum).BMC plant biology · 2025Article
- Integrated transcriptomic and metabolomic analysis reveals regulatory mechanisms underlying coloration differences in the pericarps of two Litchi cultivars.Food chemistry. Molecular sciences · 2025Article
- Transcriptome analysis reveals the mechanism of anthocyanin accumulation induced by leaf breakage in foxtail millet.BMC plant biology · 2025Article
- Integration of metabolomic and transcriptomic analyses reveals the mechanism of anthocyanin accumulation in purple leaves of Brassica juncea var. foliosa.BMC plant biology · 2025Article
- Review
- Ethylene promotes anthocyanin synthesis in 'Viviana' lily via the LvMYB5-LvERF113-LvMYB1 module.Horticulture research · 2025Article
- Ethylene increases the NaHCOPlant biotechnology journal · 2025Article
- Association analyses reveal both anthropic and environmental selective events during eggplant domestication.The Plant journal : for cell and molecular biology · 2025Article
- Reduced apple crop density enhances total polyphenol accumulation via upregulation of anthocyanidin reductase and other phenylpropanoid pathway genes.Frontiers in plant science · 2025Article
- Assessing the impacts of aminoethoxyvinylglycine and 1-methylcyclopropene on fruit drop, cracking, quality, and related transcript accumulation in 'Ambrosia' and 'Fuji' apples during on-the-tree ripening.Frontiers in plant science · 2025Article
- Article
22 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
key messageThe regulator MdERF1B in the apple (Malus × domestica) ethylene pathway mainly acts on MdMYB9 and MdMYB11 to regulate anthocyanin and proanthocyanidin accumulation. Dietary anthocyanins and proanthocyanidins (PAs) have health benefits for humans, and are associated with decreased risks of coronary heart disease and cancer. Ethylene can enhance reddening of apple (Malus × domestica), but the regulatory mechanism is poorly understood. In this study, an ethylene response factor (ERF), MdERF1B, was identified and functionally characterized. 'Orin' calli overexpressing MdERF1B were generated and then analyzed by quantitative reverse transcription-PCR. Compared with the control calli, the MdERF1B-overexpressing calli showed increased expression levels of MdACO1, MdERF1, and MdERF3 in the ethylene pathway and MdCHS, MdCHI, MdF3H, MdDFR, MdANS, MdLAR, MdANR, MdMYB9 and MdMYB11 in the flavonoid pathway. As a result, the levels of anthocyanins and PAs were significantly increased in the MdERF1B-overexpressing calli. MdERF1B interacted with MdMYB9, MdMYB1, and MdMYB11 proteins in yeast two-hybrid, pull-down, and bimolecular fluorescence complementation assays. Furthermore, in yeast one-hybrid and electrophoretic mobility shift assays, MdERF1B also bound to the promoters of MdMYB9, MdMYB1, and MdMYB11. In a luciferase reporter assay, MdERF1B mainly activated proMdMYB9 and proMdMYB11, promoting their expression levels. This was in agreement with MdERF1B's overexpression in calli, which barely affected MdMYB1 expression. Taken together, our findings provide an insight into the regulatory mechanisms in the ethylene pathway that increase anthocyanin and PA accumulation in apple.
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