ArticleAMB Express2018
Enhanced production of poly(3-hydroxybutyrate) in recombinant Escherichia coli and EDTA-microwave-assisted cell lysis for polymer recovery.
Article in AMB Express, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 47 citations in OpenAlex.
- Polyhydroxybutyrate (PHB) production from halophiles: a comprehensive review.Bioresources and bioprocessing · 2026Review
- A review on microbes mediated resource recovery and bioplastic (polyhydroxyalkanoates) production from wastewater.Microbial cell factories · 2024Review
- Physicochemical characterization of polyhydroxybutyrate (PHB) produced by the rare halophile Brachybacterium paraconglomeratum MTCC 13074.Microbial cell factories · 2024Article
- Copolymers and Blends Based on 3-Hydroxybutyrate and 3-Hydroxyvalerate Units.International journal of molecular sciences · 2023Review
- Polyhydroxy butyrate biosynthesis by Azotobacter chroococcum MTCC 3858 through groundnut shell as lignocellulosic feedstock using resource surface methodology.Scientific reports · 2023Article
- Cocoa Bean Shell: A By-Product with High Potential for Nutritional and Biotechnological Applications.Antioxidants (Basel, Switzerland) · 2023Review
- Analysis of Polyhydroxyalkanoates Granules inPolymers · 2021Article
- Biological Approaches in Polyhydroxyalkanoates Recovery.Current microbiology · 2021Review
- Biosynthesis of poly(3-hydroxybutyrate-3 Biotech · 2020Article
- RSM-GA Based Optimization of Bacterial PHA Production andBiomolecules · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Poly(3-hydroxybutyrate) (PHB) is a bacterial polymer of great commercial importance due to its properties similar to polypropylene. With an aim to develop a recombinant system for economical polymer production, PHB biosynthesis genes from Bacillus aryabhattai PHB10 were cloned in E. coli. The recombinant cells accumulated a maximum level of 6.22 g/L biopolymer utilizing glycerol in shake flasks. The extracted polymer was confirmed as PHB by GC-MS and NMR analyses. The polymer showed melting point at 171 °C, thermal stability in a temperature range of 0-140 °C and no weight loss up to 200 °C. PHB extracted from sodium hypochlorite lysed cells had average molecular weight of 143.108 kDa, polydispersity index (PDI) 1.81, tensile strength of 14.2 MPa and an elongation at break of 7.65%. This is the first report on high level polymer accumulation in recombinant E. coli solely expressing PHB biosynthesis genes from a Bacillus sp. As an alternative to sodium hypochlorite cell lysis mediated polymer extraction, the effect of combined treatment with ethylenediaminetetraacetic acid and microwave was studied which attained 93.75% yield. The polymer recovered through this method was 97.21% pure, showed 2.9-fold improvement in molecular weight and better PDI. The procedure is simple, with minimum polymer damage and more eco-friendly than the sodium hypochlorite lysis method.
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Registered trials
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