ArticleScientific reports2018
Rational metabolic-flow switching for the production of exogenous secondary metabolites in bamboo suspension cells.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 31 citations in OpenAlex.
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- In vitro bioactive metabolite production and plant regeneration of medicinal plant Ligusticum chuanxiong Hort.BMC plant biology · 2026Article
- Bioproduction ofPlant biotechnology (Tokyo, Japan) · 2025Article
- Bioproduction of raspberry ketone usingPlant biotechnology (Tokyo, Japan) · 2025Article
- Molecular Identification and Functional Characterization of a Putrescine Hydroxycinnamoyltransferase in Suspension Cells of Bamboo (Phyllostachys nigra).Applied biochemistry and biotechnology · 2025Article
- Induced accumulation of serotonin in gibberellin APlant biotechnology (Tokyo, Japan) · 2025Article
- Integrated morphophysiological, transcriptomic, and metabolomic data uncover the molecular mechanism of environmental adaptation ofFrontiers in plant science · 2025Article
- Occurrence of caffeoylquinic acids in bamboo suspension cells cultured under light.Plant biotechnology (Tokyo, Japan) · 2024Article
- Identification of a di-glucose conjugate of 4-hydroxybenzoic acid in bamboo cells expressing bacterial 4-hydroxycinnamoyl-CoA hydratase/lyase.Plant biotechnology (Tokyo, Japan) · 2024Article
- Avenanthramides, Distinctive Hydroxycinnamoyl Conjugates of Oat,Plants (Basel, Switzerland) · 2023Review
- Contribution of endophytes towards improving plant bioactive metabolites: a rescue option against red-taping of medicinal plants.Frontiers in plant science · 2023Review
- High-transcriptional activation ability of bamboo SECONDARY WALL NAC transcription factors is derived from C-terminal domain.Plant biotechnology (Tokyo, Japan) · 2022Article
- Synthetic Biology in Plants, a Boon for Coming Decades.Molecular biotechnology · 2021Review
- Plant Platforms for Efficient Heterologous Protein Production.Biotechnology and bioprocess engineering : BBE · 2021Review
- Crystal structure of barley agmatine coumaroyltransferase, an N-acyltransferase from the BAHD superfamily.Acta crystallographica. Section F, Structural biology communications · 2020Article
- Comparative transcriptome analysis to identify candidate genes involved in 2-methoxy-1,4-naphthoquinone (MNQ) biosynthesis in Impatiens balsamina L.Scientific reports · 2020Article
- Profiling of Chlorogenic Acids fromMetabolites · 2020Article
- Biosynthesis of Phenylamide Phytoalexins in Pathogen-Infected Barley.International journal of molecular sciences · 2019Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The synthetic biology-driven production of high-value plant secondary metabolites in microbial hosts has attracted extensive attention despite various challenges, including correct protein expression and limited supplies of starting materials. In contrast, plant cell cultures are rarely used for this purpose owing to their slow proliferation rates and laborious transformation processes. Here, we propose a "rational metabolic-flow switching" strategy to efficiently produce exogenous secondary metabolites using suspension-cultured bamboo (Phyllostachys nigra; Pn) cells as model production hosts. The Pn cells biosynthesise hydroxycinnamic acid amides (HCAAs) of putrescine as major secondary metabolites, which indicates that the phenylpropanoid and polyamine biosynthetic pathways are highly active and that the Pn cells may produce alternative secondary metabolites derived from those pathways. Stable transformants of Pn cells expressing agmatine coumaroyltransferase of barley (Hordeum vulgare) were generated with the expectation of metabolic-flow switching from HCAAs of putrescine to those of agmatine. In the recombinant Pn cells, the levels of HCAAs of putrescine decreased and the HCAAs of agmatine were produced instead. The production titre of the major product, p-coumaroylagmatine, reached approximately 360 mg/L, providing a proof-of-concept for the usefulness of "rational metabolic-flow switching" in synthetic biology using plant cell hosts.
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