Evidence map›Paper›PMID 30181605›Full record

ArticleNature communications2018

Local enrichment of HP1alpha at telomeres alters their structure and regulation of telomere protection.

Tracy T Chow, Xiaoyu Shi, Jen-Hsuan Wei, Juan Guan, Guido Stadler, Bo Huang, Elizabeth H Blackburn

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
2.2field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 26 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. Review
  11. Telomeres and Cancer.Life (Basel, Switzerland) · 2021
    Review
  12. Regulation of Gene Expression by Telomere Position Effect.International journal of molecular sciences · 2021
    Review
  13. Review
  14. Review
  15. Article
  16. Review
  17. Epigenetics and genome stability.Mammalian genome : official journal of the International Mammalian Genome Society · 2020
    Review
  18. Review
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Tracy T ChowDepartment of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, 94143, USA.ORCID http://orcid.org/0000-0002-1690-8035
Xiaoyu ShiDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA, 94143, USA.ORCID http://orcid.org/0000-0002-9634-2659
Jen-Hsuan WeiDepartment of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, 94143, USA.
Juan GuanDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA, 94143, USA.
Guido StadlerBerkeley Lights Inc, Emeryville, CA, 94608, USA.
Bo HuangDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA, 94143, USA.ORCID http://orcid.org/0000-0003-1704-4141
Elizabeth H BlackburnDepartment of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, 94143, USA. Elizabeth.Blackburn@ucsf.edu.ORCID http://orcid.org/0000-0002-4885-0544
University of California, San Francisco · USHoward Hughes Medical Institute · USSalk Institute for Biological Studies · US

Funding

Synergism of telomere maintenance and telomerase with cancer-promoting signalingR01CA096840 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI BLACKBURN, ELIZABETH H · 2002 to 2016
$4.8M
Elucidating How Primary Cilia Regulate Hedgehog Signaling by Super-Resolution MicroscopyK99GM126136 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI SHI, XIAOYU · 2018 to 2019
$128k
NCI NIH HHS R01 CA096840NIGMS NIH HHS K99 GM126136
6 · The paper itself

Abstract

Enhanced telomere maintenance is evident in malignant cancers. While telomeres are thought to be inherently heterochromatic, detailed mechanisms of how epigenetic modifications impact telomere protection and structures are largely unknown in human cancers. Here we develop a molecular tethering approach to experimentally enrich heterochromatin protein HP1α specifically at telomeres. This results in increased deposition of H3K9me3 at cancer cell telomeres. Telomere extension by telomerase is attenuated, and damage-induced foci at telomeres are reduced, indicating augmentation of telomere stability. Super-resolution STORM imaging shows an unexpected increase in irregularity of telomeric structure. Telomere-tethered chromo shadow domain (CSD) mutant I165A of HP1α abrogates both the inhibition of telomere extension and the irregularity of telomeric structure, suggesting the involvement of at least one HP1α-ligand in mediating these effects. This work presents an approach to specifically manipulate the epigenetic status locally at telomeres to uncover insights into molecular mechanisms underlying telomere structural dynamics.

Indexed as

Cell Line, TumorChromatinChromobox Protein Homolog 5Chromosomal Proteins, Non-HistoneDNA DamageHeterochromatinHistonesHumansLysineMicroscopyMutationProtein DomainsRecombinant Fusion ProteinsTelomereTelomeric Repeat Binding Protein 1Telomeric Repeat Binding Protein 2CBX5 protein, humanChromatinChromobox Protein Homolog 5Chromosomal Proteins, Non-HistoneHeterochromatinHistonesLysineRecombinant Fusion ProteinsTelomeric Repeat Binding Protein 1Telomeric Repeat Binding Protein 2TERF2 protein, human

Identifiers

PMID30181605
PMCPMC6123478
OpenAlexW2949484687

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.