Evidence map›Paper›PMID 30181601›Full record

ArticleScientific reports2018

Non-targeted metabolic profiling of BW312 Hordeum vulgare semi dwarf mutant using UHPLC coupled to QTOF high resolution mass spectrometry.

Claire Villette, Julie Zumsteg, Hubert Schaller, Dimitri Heintz

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
3.7field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 23 citations in OpenAlex.

  1. Article
  2. Article
  3. TheInternational journal of molecular sciences · 2022
    Article
  4. Article
  5. Article
  6. Heterologous microProtein expression identifies LITTLE NINJA, a dominant regulator of jasmonic acid signaling.Proceedings of the National Academy of Sciences of the United States of America · 2020
    Article
  7. Systems Metabolic Alteration in a Semi-Dwarf Rice Mutant Induced byInternational journal of molecular sciences · 2020
    Article
  8. Xenobiotics metabolization in Salix alba leaves uncovered by mass spectrometry imaging.Metabolomics : Official journal of the Metabolomic Society · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Claire VillettePlant Imaging and Mass Spectrometry, Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 12 rue du Général Zimmer, 67084, Strasbourg, France. claire.villette@ibmp-cnrs.unistra.fr.
Julie ZumstegPlant Imaging and Mass Spectrometry, Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 12 rue du Général Zimmer, 67084, Strasbourg, France.
Hubert SchallerPlant Isoprenoid Biology, Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 12 rue du Général Zimmer, 67084, Strasbourg, France.
Dimitri HeintzPlant Imaging and Mass Spectrometry, Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 12 rue du Général Zimmer, 67084, Strasbourg, France.
Centre National de la Recherche Scientifique · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Barley (Hordeum vulgare) is the fourth crop cultivated in the world for human consumption and animal feed, making it important to breed healthy and productive plants. Among the threats for barley are lodging, diseases, and pathogens. To avoid lodging, dwarf and semi-dwarf mutants have been selected through breeding processes. Most of these mutants are affected on hormonal biosynthesis or signalling. Here, we present the metabolic characterization of a brassinosteroid insensitive semi-dwarf mutant, BW312. The hormone profile was determined through a targeted metabolomics analysis by UHPLC-triple quadrupole-MS/MS, showing an induction of gibberellic acid and jasmonic acid in the semi-dwarf mutant. A non-targeted metabolomics analysis by UHPLC-QTOF-MS/MS revealed a differential metabolic profile, with 16 and 9 metabolites showing higher intensities in the mutant and wild-type plants respectively. Among these metabolites, azelaic acid was identified. Gibberellic acid, jasmonic acid, and azelaic acid are involved in pathogen resistance, showing that this semi-dwarf line has an enhanced basal pathogen resistance in absence of pathogens, and therefore is of interest in breeding programs to fight against lodging, but also probably to increase pathogen resistance.

Indexed as

MetabolomeBrassinosteroidsChromatography, High Pressure LiquidCyclopentanesDicarboxylic AcidsGibberellinsHordeumMetabolomicsMutationOxylipinsPhenotypePlant BreedingPlant ProteinsTandem Mass Spectrometryazelaic acidBrassinosteroidsCyclopentanesDicarboxylic Acidsgibberellic acidGibberellinsjasmonic acidOxylipinsPlant Proteins

Identifiers

PMID30181601
PMCPMC6123459
OpenAlexW2889500543

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.