ArticleNature communications2018
Engineered bidirectional promoters enable rapid multi-gene co-expression optimization.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed.
- Advances in Key Genetic Elements and Strategies for High-Yield Heterologous Protein Expression inJournal of fungi (Basel, Switzerland) · 2026Review
- A modular single- and dual-gene expression toolkit for Kluyveromyces marxianus.World journal of microbiology & biotechnology · 2026Article
- Engineering bidirectional chloroplast promoters for tunable co-expression of multiple genes in microalgae (Chlamydomonas reinhardtii).Communications biology · 2026Article
- Fed-Batch Operational Strategies for Efficient and Robust Pichia Pastoris Cultivations Achieving High Cell Densities.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Retrofitting of AOX1 Promoter-Based Pichia pastoris Expression Strains for Methanol-Independent Protein Production.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Promoter engineering for enhanced 3-hydroxypropionic acid production inSynthetic and systems biotechnology · 2025Article
- Through the lens of bioenergy crops: advances, bottlenecks, and promises of plant engineering.The Plant journal : for cell and molecular biology · 2025Review
- Unraveling the regulatory dynamics of bidirectional promoters for modulating gene co-expression and metabolic flux in Saccharomyces cerevisiae.Nucleic acids research · 2025Article
- A toolkit for facilitating markerless integration of expression cassettes in Komagataella phaffii via CRISPR/Cas9.Microbial cell factories · 2025Article
- Plant engineering: advances, bottlenecks, and promise.The Plant journal : for cell and molecular biology · 2025Article
- Engineering artificial cross-species promoters with different transcriptional strengths.Synthetic and systems biotechnology · 2025Article
- Genomic Analysis and Functional Validation of Bidirectional Promoters in Medaka (International journal of molecular sciences · 2024Article
- Enhancing D-lactic acid production by optimizing the expression of D-LDH gene in methylotrophic yeast Komagataella phaffii.Biotechnology for biofuels and bioproducts · 2024Article
- Engineering signalling pathways in mammalian cells.Nature biomedical engineering · 2024Review
- The Plasma Membrane H+-ATPase Promoter Driving the Expression ofJournal of fungi (Basel, Switzerland) · 2024Article
- Bi-directionalized promoter systems allow methanol-free production of hard-to-express peroxygenases with Komagataella Phaffii.Microbial cell factories · 2024Article
- Comparison of CRISPR-MAD7 and CRISPR-Cas9 for Gene Disruptions inJournal of fungi (Basel, Switzerland) · 2024Article
- Identification and application of a strong bidirectionalEngineering microbiology · 2024Article
- Combinatorial optimization of gene expression through recombinase-mediated promoter and terminator shuffling in yeast.Nature communications · 2024Article
- Plant Engineering to Enable Platforms for Sustainable Bioproduction of Terpenoids.Methods in molecular biology (Clifton, N.J.) · 2024Review
3 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
- Erratum issued
Authors and funding
16 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Numerous synthetic biology endeavors require well-tuned co-expression of functional components for success. Classically, monodirectional promoters (MDPs) have been used for such applications, but MDPs are limited in terms of multi-gene co-expression capabilities. Consequently, there is a pressing need for new tools with improved flexibility in terms of genetic circuit design, metabolic pathway assembly, and optimization. Here, motivated by nature's use of bidirectional promoters (BDPs) as a solution for efficient gene co-expression, we generate a library of 168 synthetic BDPs in the yeast Komagataella phaffii (syn. Pichia pastoris), leveraging naturally occurring BDPs as a parts repository. This library of synthetic BDPs allows for rapid screening of diverse expression profiles and ratios to optimize gene co-expression, including for metabolic pathways (taxadiene, β-carotene). The modular design strategies applied for creating the BDP library could be relevant in other eukaryotic hosts, enabling a myriad of metabolic engineering and synthetic biology applications.
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