Evidence map›Paper›PMID 30180933›Full record

ArticleeLife2018

A two-hybrid antibody micropattern assay reveals specific

Cindy Dirscherl, Zeynep Hein, Venkat Raman Ramnarayan, Catherine Jacob-Dolan, Sebastian Springer

Open access · goldAbstract read
In one paragraph

Article in eLife, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.8field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Homotypic and heterotypicCurrent research in immunology · 2022
    Review
  5. Article
  6. Open MHC Class I Conformers: A Look through the Looking Glass.International journal of molecular sciences · 2021
    Review
  7. Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Cindy DirscherlDepartment of Life Sciences and Chemistry, Jacobs University, Bremen, Germany.ORCID 0000-0002-8973-2835
Zeynep HeinDepartment of Life Sciences and Chemistry, Jacobs University, Bremen, Germany.ORCID 0000-0002-6335-8961
Venkat Raman RamnarayanDepartment of Life Sciences and Chemistry, Jacobs University, Bremen, Germany.
Catherine Jacob-DolanDepartment of Life Sciences and Chemistry, Jacobs University, Bremen, Germany.
Sebastian SpringerDepartment of Life Sciences and Chemistry, Jacobs University, Bremen, Germany.ORCID 0000-0002-5527-6149
Constructor University · DE

Funding

Bundesministerium für Bildung und Forschung 031A153ADeutsche Forschungsgemeinschaft SP583/7-2Tönjes Vagt Foundation XXXII
6 · The paper itself

Abstract

We demonstrate a two-hybrid assay based on antibody micropatterns to study protein-protein interactions at the cell surface of major histocompatibility complex class I (MHC I) proteins. Anti-tag and conformation-specific antibodies are used for individual capture of specific forms of MHC I proteins that allow for location- and conformation-specific analysis by fluorescence microscopy. The assay is used to study the

Indexed as

Two-Hybrid System TechniquesAnimalsAntibodiesCell MembraneGreen Fluorescent ProteinsHistocompatibility Antigens Class IMicePeptidesProtein MultimerizationAntibodiesGreen Fluorescent ProteinsHistocompatibility Antigens Class IPeptidescell biologyendocytosisimmunologyin cis interactionsinflammationmajor histocompatibility complex moleculesmembrane proteinsprotein micropatterns

Identifiers

PMID30180933
PMCPMC6125123
OpenAlexW2891707110

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.