Evidence map›Paper›PMID 30180879›Full record

ArticleParasites & vectors2018

Sequence and structural variation in the genome of the Biomphalaria glabrata embryonic (Bge) cell line.

Nicolas J Wheeler, Nathalie Dinguirard, Joshua Marquez, Adrian Gonzalez, Mostafa Zamanian, Timothy P Yoshino, Maria G Castillo

Erratum issuedAbstract read
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In one paragraph

Article in Parasites & vectors, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Carnosine Synthase (Antioxidants (Basel, Switzerland) · 2024
    Article
  2. Article
  3. Review
  4. Review
  5. Mobilizing molluscan models and genomes in biology.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021
    Article
  6. Mitochondrial genome ofCurrent research in parasitology & vector-borne diseases · 2021
    Article
  7. Article
  8. Gene drives for schistosomiasis transmission control.PLoS neglected tropical diseases · 2019
    Review
  9. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Nicolas J WheelerDepartment of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI, USA.
Nathalie DinguirardDepartment of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI, USA.
Joshua MarquezDepartment of Biology, New Mexico State University, Las Cruces, NM, USA.
Adrian GonzalezDepartment of Biology, New Mexico State University, Las Cruces, NM, USA.
Mostafa ZamanianDepartment of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI, USA.
Timothy P YoshinoDepartment of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI, USA.
Maria G CastilloDepartment of Biology, New Mexico State University, Las Cruces, NM, USA. mcastill@nmsu.edu.

Funding

Characterization of Thioester-containing Proteins (TEPs) in the snail Biomphalaria glabrataSC2AI133645 · NIAID · NEW MEXICO STATE UNIVERSITY LAS CRUCES · PI CASTILLO, MARIA G · 2017 to 2019
$440k
National Institute of Allergy and Infectious Diseases 1SC2AI133645-0NCGR-NM-INBRE NMINBRE_M.Castillo_May_2016NIAID NIH HHS SC2 AI133645
6 · The paper itself

Abstract

backgroundThe aquatic pulmonate snail Biomphalaria glabrata is a significant vector and laboratory host for the parasitic flatworm Schistosoma mansoni, an etiological agent for the neglected tropical disease schistosomiasis. Much is known regarding the host-parasite interactions of these two organisms, and the B. glabrata embryonic (Bge) cell line has been an invaluable resource in these studies. The B. glabrata BB02 genome sequence was recently released, but nothing is known of the sequence variation between this reference and the Bge cell genome, which has likely accumulated substantial genetic variation in the ~50 years since its isolation.

resultsHere, we report the genome sequence of our laboratory subculture of the Bge cell line (designated Bge3), which we mapped to the B. glabrata BB02 reference genome. Single nucleotide variants (SNVs) were predicted and focus was given to those SNVs that are most likely to affect the structure or expression of protein-coding genes. Furthermore, we have highlighted and validated high-impact SNVs in genes that have often been studied using Bge cells as an in vitro model, and other genes that may have contributed to the immortalization of this cell line. We also resolved representative karyotypes for the Bge3 subculture, which revealed a mixed population exhibiting substantial aneuploidy, in line with previous reports from other Bge subcultures.

conclusionsThe Bge3 genome differs from the B. glabrata BB02 reference genome in both sequence and structure, and these are likely to have significant biological effects. The availability of the Bge3 genome sequence, and an awareness of genomic differences with B. glabrata, will inform the design of experiments to understand gene function in this unique in vitro snail cell model. Additionally, this resource will aid in the development of new technologies and molecular approaches that promise to reveal more about this schistosomiasis-transmitting snail vector.

Indexed as

GenomeAnimalsBiomphalariaCell LineDisease VectorsEmbryo, NonmammalianKaryotypingPolymorphism, Single NucleotideSchistosoma mansoniBgeBiomphalaria glabrataGenome sequenceKaryotypeSchistosoma mansoniVariant calling

Identifiers

PMID30180879
PMCPMC6122571

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.