ArticleThe Journal of cell biology1986
Clathrin-coated vesicular transport of secretory proteins during the formation of ACTH-containing secretory granules in AtT20 cells.
Article in The Journal of cell biology, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
79 citing papers in PubMed, 217 citations in OpenAlex.
- BEACH domain proteins function as cargo-sorting adaptors in secretory and endocytic pathways.The Journal of cell biology · 2024Article
- Liquid-liquid phase separation facilitates the biogenesis of secretory storage granules.The Journal of cell biology · 2022Article
- Coding variants identified in patients with diabetes alter PICK1 BAR domain function in insulin granule biogenesis.The Journal of clinical investigation · 2022Article
- Quantitative mass spectrometry for human melanocortin peptides in vitro and in vivo suggests prominent roles for β-MSH and desacetyl α-MSH in energy homeostasis.Molecular metabolism · 2018Article
- POMC: The Physiological Power of Hormone Processing.Physiological reviews · 2018Review
- Role of clathrin in dense core vesicle biogenesis.Molecular biology of the cell · 2017Article
- 60 YEARS OF POMC: Biosynthesis, trafficking, and secretion of pro-opiomelanocortin-derived peptides.Journal of molecular endocrinology · 2016Review
- AP-1A controls secretory granule biogenesis and trafficking of membrane secretory granule proteins.Traffic (Copenhagen, Denmark) · 2014Article
- Souffle/Spastizin controls secretory vesicle maturation during zebrafish oogenesis.PLoS genetics · 2014Article
- Role of adaptor proteins in secretory granule biogenesis and maturation.Frontiers in endocrinology · 2013Article
- AP-1 and clathrin are essential for secretory granule biogenesis in Drosophila.Molecular biology of the cell · 2011Article
- Vacuolization of mucolipidosis type II mouse exocrine gland cells represents accumulation of autolysosomes.Molecular biology of the cell · 2011Article
- Secretory granule membrane protein recycles through multivesicular bodies.Traffic (Copenhagen, Denmark) · 2010Article
- How peptide hormone vesicles are transported to the secretion site for exocytosis.Molecular endocrinology (Baltimore, Md.) · 2008Review
- Discovery and progress in our understanding of the regulated secretory pathway in neuroendocrine cells.Histochemistry and cell biology · 2008Review
- Not all secretory granules are created equal: Partitioning of soluble content proteins.Molecular biology of the cell · 2006Article
- GGA function is required for maturation of neuroendocrine secretory granules.The EMBO journal · 2006Article
- Adaptor protein complex 1 mediates the transport of lysosomal proteins from a Golgi-like organelle to peripheral vacuoles in the primitive eukaryote Giardia lamblia.Molecular biology of the cell · 2004Article
- Signaling mediated by the cytosolic domain of peptidylglycine alpha-amidating monooxygenase.Molecular biology of the cell · 2001Article
- A phosphorylation site regulates sorting of the vesicular acetylcholine transporter to dense core vesicles.The Journal of cell biology · 2000Article
19 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have studied by electron microscopy and immunocytochemistry the formation of secretory granules containing adrenocorticotropic hormone (ACTH) in murine pituitary cells of the AtT20 line. The first compartment in which condensed secretory protein appears is a complex reticular network at the extreme trans side of the Golgi stacks beyond the TPPase-positive cisternae. Condensed secretory protein accumulates in dilated regions of this trans Golgi network. Examination of en face and serial sections revealed that "condensing vacuoles" are in fact dilations of the trans Golgi network and not detached vacuoles. Only after presumptive secretory granules have reached an advanced stage of morphological maturation do they detach from the trans Golgi network. Frequently both the dilations of the trans Golgi network containing condensing secretory protein and the detached immature granules in the peri-Golgi region have surface coats which were identified as clathrin by immunocytochemistry. Moreover both are the site of budding (or fusion) of coated vesicles, some of which contain condensed secretory protein. The mature granules below the plasma membrane do not, however, have surface coats. Immunoperoxidase labeling with an antiserum specific for ACTH and its precursor polypeptide confirmed that many of the coated vesicles associated with the trans Golgi network contain ACTH. The involvement of the trans Golgi network and coated vesicles in the formation of secretory granules is discussed.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.