ArticleAnalytical chemistry2018
Ultra-High Pressure (>30,000 psi) Packing of Capillary Columns Enhancing Depth of Shotgun Proteomic Analyses.
Article in Analytical chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed.
- Comparison of ultrahigh- and low-pressure packing methods for preparation of 50 cm long capillary UHPLC columns.Journal of chromatography. A · 2026Article
- Limited Impact of Column Chemistry and Length on Proteome Coverage Under High-Speed DIA.Molecular & cellular proteomics : MCP · 2026Article
- Regarding Emitter Positioning for Nanoflow Electrospray Ionization with a High-Capacity Inlet Capillary.Journal of the American Society for Mass Spectrometry · 2026Article
- Proteomic and Lipidomic Atlas of Gut-Associated Lymph and Venous Depots in Female Piglets.Arteriosclerosis, thrombosis, and vascular biology · 2026Article
- Orthogonal chemical genomics approaches reveal genomic targets for increasing anaerobic chemical tolerance inmSystems · 2026Article
- Atlas of lysine acetylation in the mouse.bioRxiv : the preprint server for biology · 2026Article
- Dynamic Quadrupole Selection to Associate Precursor Masses with MS/MS Products in Data-Independent Acquisition.Journal of the American Society for Mass Spectrometry · 2025Article
- SynchroSep-MS: Parallel LC Separations for Multiplexed Proteomics.Journal of the American Society for Mass Spectrometry · 2025Article
- Resolving fibrosis by stimulating HSC-dependent extracellular matrix degradation.Science translational medicine · 2025Article
- IFIT3 RNA-binding activity promotes influenza A virus infection and translation efficiency.Journal of virology · 2025Article
- Machine learning reveals genes impacting oxidative stress resistance across yeasts.Nature communications · 2025Article
- SPE-CZE-MS Quantifies Zeptomole Amounts of Phosphorylated Peptides.Journal of proteome research · 2025Article
- Technical Evaluation of Plasma Proteomics Technologies.Journal of proteome research · 2025Article
- IFIT3 RNA-binding activity promotes influenza A virus infection and translation efficiency.bioRxiv : the preprint server for biology · 2025Article
- Boosting the Sensitivity of Quantitative Single-Cell Proteomics with Infrared-Tandem Mass Tags.Journal of proteome research · 2025Article
- Altering translation allows E. coli to overcome G-quadruplex stabilizers.Nucleic acids research · 2025Article
- Article
- Deep Learning Predicts Non-Normal Transmission Distributions in High-Field Asymmetric Waveform Ion Mobility (FAIMS) Directly from Peptide Sequence.Analytical chemistry · 2025Article
- Triacylglycerol mobilization underpins mitochondrial stress recovery.Nature cell biology · 2025Article
- Global cellular proteo-lipidomic profiling of diverse lysosomal storage disease mutants using nMOST.Science advances · 2025Article
6 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors.
Funding
Abstract
Extreme sample complexity is an inherent challenge in shotgun proteomics that positions quality of chromatographic separations as one of the key determinants of attainable proteome coverage. In search of better separations, macroscopic physical characteristics of capillary columns, i.e., length and properties of stationary phase particles, are typically considered and optimized, while significance of packing bed morphology is frequently underappreciated. Here, we describe a technology that enables packing of capillary columns at excess of 30,000 psi and demonstrate that such columns exhibit reduced backpressure and remarkably reproducible chromatographic performance, improved on average by 23%. These enhancements afford up to 35% increase in the depth of commonplace bottom-up proteomic analyses, owning to augmented sensitivity and resolution of peptide separations and improvements in spectral quality. Our findings strongly corroborate advantages of ultra-high pressure packing of capillary columns for diverse shotgun proteomic workflows.
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