ArticleACS applied materials & interfaces2018
Membraneless Compartmentalization Facilitates Enzymatic Cascade Reactions and Reduces Substrate Inhibition.
Article in ACS applied materials & interfaces, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed, 112 citations in OpenAlex.
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- Control of encounter kinetics by chemically active droplets.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Substrate Exclusion Greenlights Physical Autocatalysis of Enzyme Activity in Membraneless Proto-Organelles.Biomacromolecules · 2025Article
- Enhancement of enzymatic activity by biomolecular condensates through pH buffering.Nature communications · 2025Article
- Structured protein domains enter the spotlight: modulators of biomolecular condensate form and function.Trends in biochemical sciences · 2025Review
- Regulation of enzymatic reactions by chemical composition of peptide biomolecular condensates.Communications chemistry · 2024Article
- Dynamic control of DNA condensation.Nature communications · 2024Article
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- Active learning of the thermodynamics-dynamics trade-off in protein condensates.Science advances · 2024Article
- Improving pathway prediction accuracy of constraints-based metabolic network models by treating enzymes as microcompartments.Synthetic and systems biotechnology · 2023Article
- Label-free autofluorescence lifetime reveals the structural dynamics of ataxin-3 inside droplets formed via liquid-liquid phase separation.Scientific reports · 2023Article
- A spatially resolved elemental nanodomain organization within acidocalcisomes inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Crowding-induced phase separation and gelling by co-condensation of PEG in NPM1-rRNA condensates.Biophysical journal · 2023Article
- Dynamic RNA synthetic biology: new principles, practices and potential.RNA biology · 2023Review
- Water-in-water droplet microfluidics: A design manual.Biomicrofluidics · 2022Review
- Chemical Kinetics and Mass Action in Coexisting Phases.Journal of the American Chemical Society · 2022Article
- Frustration and the Kinetic Repartitioning Mechanism of Substrate Inhibition in Enzyme Catalysis.The journal of physical chemistry. B · 2022Article
- Cascade reaction networks within audible sound induced transient domains in a solution.Nature communications · 2022Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Living cells possess membraneless organelles formed by liquid-liquid phase separation. With the aim of better understanding the general functions of membraneless microcompartments, this paper constructs acellular multicompartment reaction systems using an aqueous multiphase system. Membraneless coacervate droplets are placed within a molecularly crowded environment, where a larger dextran (DEX) droplet is submerged in a polyethylene glycol (PEG) solution. The coacervate droplets are capable of sequestering reagents and enzymes with a long retention time, and demonstrate multistep cascading reactions through the liquid-liquid interfaces. The ability to change phase dynamics is also demonstrated through salt-mediated dissolution of coacervate droplets, which leads to the release and mixing of separately sequestered reagents and enzymes. Finally, as phase-separated materials in membraneless organelles are often substrates and substrate analogues for the enzymes sequestered or excluded in the organelles, this paper explores the interaction between DEX and dextranase, an enzyme that hydrolyzes DEX. The results reveal that dextranase suffers from substrate inhibition when partitioned directly in a DEX phase but that this inhibition can be mitigated and reactions greatly accelerated by compartmentalization of dextranase inside a coacervate droplet that is adjacent to, but phase-separated from, the DEX phase. The insight that compartmentalization of enzymes can accelerate reactions by mitigating substrate inhibition is particularly novel and is an example where artificial membraneless organelle-like systems may provide new insights into physiological cell functions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.