ReviewChembiochem : a European journal of chemical biology2019
Enzyme Fusions in Biocatalysis: Coupling Reactions by Pairing Enzymes.
Review in Chembiochem : a European journal of chemical biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Employing Nanotechnology to Enhance Ethanol Production in Two Synechococcus elongatus Strains.Chembiochem : a European journal of chemical biology · 2026Article
- Elevating Haloperoxidase Expression in Escherichia coli through Fusion with a Formate Oxidase.Chembiochem : a European journal of chemical biology · 2026Article
- Engineering fusion enzymes for enhanced catalysis and stability: strategies and biotechnological applications.Molecular biology reports · 2026Review
- Enhanced Catalytic Performance of P450 Monooxygenase CYP11B1 by Constructing Fusion Protein with Preferred Reductase Domain and Linker.Applied biochemistry and biotechnology · 2026Article
- A protein-fragment complementation assay to quantify synthetic protein scaffold efficiency.Synthetic biology (Oxford, England) · 2026Article
- Discovery and engineering of bifunctional enzymes for lignocellulose degradation: Metagenomic and computational approaches.Biotechnology reports (Amsterdam, Netherlands) · 2025Review
- Chemoenzymatic synthesis.Communications chemistry · 2025Article
- Structural basis of FpGalNase and its combination with FpGalNAcDeAc for efficient A-to-O blood group conversion.Experimental hematology & oncology · 2025Article
- Genetically Engineered Liposwitch-Based Nanomaterials.Biomacromolecules · 2024Article
- Engineering a Bifunctional Fusion Purine/Pyrimidine Nucleoside Phosphorylase for the Production of Nucleoside Analogs.Biomolecules · 2024Article
- Expanding the toolbox of Baeyer-Villiger and flavin monooxygenase biocatalysts for the enantiodivergent green synthesis of sulfoxides.Green chemistry : an international journal and green chemistry resource : GC · 2024Article
- Engineering Electron Transfer Pathway of Cytochrome P450s.Molecules (Basel, Switzerland) · 2024Review
- Multifunctional Biocatalysts for Organic Synthesis.Journal of the American Chemical Society · 2024Review
- Cytochrome P450 for environmental remediation: catalytic mechanism, engineering strategies and future prospects.World journal of microbiology & biotechnology · 2023Review
- Bicyclo[3.2.0]carbocyclic Molecules and Redox Biotransformations: The Evolution of Closed-Loop Artificial Linear Biocatalytic Cascades and Related Redox-Neutral Systems.Molecules (Basel, Switzerland) · 2023Review
- Multi-Enzymatic Cascade for Efficient Deracemization of dl-Pantolactone into d-Pantolactone.Molecules (Basel, Switzerland) · 2023Article
- Anaerobic demethylation of guaiacyl-derived monolignols enabled by a designed artificial cobalamin methyltransferase fusion enzyme.RSC advances · 2023Article
- Engineering Catalytically Self-Sufficient P450s.Biochemistry · 2023Review
- Dual-function transaminases with hybrid nanoflower for the production of value-added chemicals from biobased levulinic acid.Frontiers in bioengineering and biotechnology · 2023Article
- Metabolic engineering of Rhodotorula toruloides for resveratrol production.Microbial cell factories · 2022Article
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Authors and funding
2 authors.
Funding
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Abstract
One approach to bringing enzymes together for multienzyme biocatalysis is genetic fusion. This enables the production of multifunctional enzymes that can be used for whole-cell biotransformations or for in vitro (cascade) reactions. In some cases and in some aspects, such as expression and conversions, the fused enzymes outperform a combination of the individual enzymes. In contrast, some enzyme fusions are greatly compromised in activity and/or expression. In this Minireview, we give an overview of studies on fusions between two or more enzymes that were used for biocatalytic applications, with a focus on oxidative enzymes. Typically, the enzymes are paired to facilitate cofactor recycling or cosubstrate supply. In addition, different linker designs are briefly discussed. Although enzyme fusion is a promising tool for some biocatalytic applications, future studies could benefit from integrating the findings of previous studies in order to improve reliability and effectiveness.
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