Evidence map›Paper›PMID 30178168›Full record

ArticleJournal of industrial microbiology & biotechnology2018

Functional metabolomics approach reveals the reduced biosynthesis of fatty acids and TCA cycle is required for pectinase activity in Bacillus licheniformis.

Yi Guan, Di Yin, Xi Du, Xiuyun Ye

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of industrial microbiology & biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
2.3field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 12 citations in OpenAlex.

  1. Isocitrate dehydrogenase of Bacillus cereus is involved in biofilm formation.World journal of microbiology & biotechnology · 2021
    Article
  2. Harnessing microbial metabolomics for industrial applications.World journal of microbiology & biotechnology · 2019
    Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Yi GuanFujian Key Laboratory of Marine Enzyme Engineering, College of Biological Sciences and Technology, Fuzhou University, No. 2 Xue Yuan Road, Fuzhou, 350108, Fujian, China. gy@fzu.edu.cn.ORCID http://orcid.org/0000-0003-3695-3973
Di YinFujian Key Laboratory of Marine Enzyme Engineering, College of Biological Sciences and Technology, Fuzhou University, No. 2 Xue Yuan Road, Fuzhou, 350108, Fujian, China.
Xi DuFujian Key Laboratory of Marine Enzyme Engineering, College of Biological Sciences and Technology, Fuzhou University, No. 2 Xue Yuan Road, Fuzhou, 350108, Fujian, China.
Xiuyun YeFujian Key Laboratory of Marine Enzyme Engineering, College of Biological Sciences and Technology, Fuzhou University, No. 2 Xue Yuan Road, Fuzhou, 350108, Fujian, China. xiuyunye@fzu.edu.cn.
Fuzhou University · CN

Funding

Foundation of Marine Bioenzyme Engineering Innovation Service Platform 2014FJPT02Fuzhou University XRC-1661National Natural Science Foundation of China 31701844Natural Science Foundation of Fujian Province 2018J05057
6 · The paper itself

Abstract

Increase of pectinase activity is especially important in fermentation industry. Understanding of the metabolic mechanisms can find metabolic modulation approach to promote high yield of pectinase. Higher activity of pectinase was detected in DY1 than DY2, two strains of Bacillus licheniformis. GC-MS-based metabolomics identified differential metabolome of DY2 compared with DY1, characterizing the increased TCA cycle and biosynthesis of fatty acids. Elevated activity of pyruvate dehydrogenase (PDH), α-ketoglutaric dehydrogenase (KGDH) and succinate dehydrogenase (SDH) showed global elevation of carbon metabolism, which is consistent with the result that lowers glucose in DY2 than DY1. Inhibitors malonate, furfural and triclosan, of PDH, SDH and biosynthesis of fatty acids, promoted pectinase activity, where triclosan increased pectinase activity by 179%. These results indicate that functional metabolomics is an effective approach to understand metabolic mechanisms of fermentation production and provides clues to develop new methods for changing bacterial physiology and production.

Indexed as

Citric Acid CycleMetabolomicsAldehyde OxidoreductasesBacillus licheniformisFatty AcidsFermentationFuraldehydeGas Chromatography-Mass SpectrometryGlucoseKetoglutarate Dehydrogenase ComplexMetabolomePolygalacturonasePyruvate Dehydrogenase ComplexSuccinate DehydrogenaseAldehyde OxidoreductasesFatty AcidsFuraldehydeGlucoseKetoglutarate Dehydrogenase ComplexPolygalacturonasePyruvate Dehydrogenase ComplexSuccinate DehydrogenaseBacillus licheniformisBiosynthesis of fatty acidsFunctional metabolomicsPectinaseTCA cycle

Identifiers

PMID30178168
OpenAlexW2889124834

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.