ArticleScientific reports2018
Regulating exopolysaccharide gene wcaF allows control of Escherichia coli biofilm formation.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 41 citations in OpenAlex.
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- Targeting bacterial biofilm-related genes with nanoparticle-based strategies.Frontiers in microbiology · 2024Review
- Driving forces shaping the microbial ecology in meat packing plants.Frontiers in microbiology · 2023Review
- Identification of differences in gene expression implicated in the Adherent-InvasiveFrontiers in cellular and infection microbiology · 2023Article
- Synthetic phosphoethanolamine-modified oligosaccharides reveal the importance of glycan length and substitution in biofilm-inspired assemblies.Nature communications · 2022Article
- Bioactive properties of ZnO nanoparticles synthesized using3 Biotech · 2022Article
- Light-regulated gene expression in Bacteria: Fundamentals, advances, and perspectives.Frontiers in bioengineering and biotechnology · 2022Review
- Epidemiologic and molecular characterization of β-lactamase-producing multidrug-resistant uropathogenic Escherichia coli isolated from asymptomatic hospitalized patients.International microbiology : the official journal of the Spanish Society for Microbiology · 2022Article
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- Insights into the structure of Escherichia coli outer membrane as the target for engineering microbial cell factories.Microbial cell factories · 2021Review
- Label-free quantitative proteomic analysis of the inhibition effect of Lactobacillus rhamnosus GG on Escherichia coli biofilm formation in co-culture.Proteome science · 2021Article
- Pyruvate Substitutions on Glycoconjugates.International journal of molecular sciences · 2019Review
- Identification of the Functional Roles of Six Key Proteins in the Biosynthesis of Enterobacteriaceae Colanic Acid.Biochemistry · 2019Article
- Controlling biofilms using synthetic biology approaches.Biotechnology advancesReview
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While biofilms are known to cause problems in many areas of human health and the industry, biofilms are important in a number of engineering applications including wastewater management, bioremediation, and bioproduction of valuable chemicals. However, excessive biofilm growth remains a key challenge in the use of biofilms in these applications. As certain amount of biofilm growth is required for efficient use of biofilms, the ability to control and maintain biofilms at desired thickness is vital. To this end, we developed synthetic gene circuits to control E. coli MG1655 biofilm formation by using CRISPRi/dCas9 to regulate a gene (wcaF) involved in the synthesis of colanic acid (CA), a key polysaccharide in E. coli biofilm extracellular polymeric substance (EPS). We showed that the biofilm formation was inhibited when wcaF was repressed and the biofilms could be maintained at a different thickness over a period of time. We also demonstrated that it is also possible to control the biofilm thickness spatially by inhibiting wcaF gene using a genetic light switch. The results demonstrate that the approach has great potential as a new means to control and maintain biofilm thickness in biofilm related applications.
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Registered trials
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