ArticleBMC veterinary research2018
Specific detection of Muscovy duck parvovirus infection by TaqMan-based real-time PCR assay.
Article in BMC veterinary research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 21 citations in OpenAlex.
- A Multiplex TaqMan-MGB qPCR Assay for Rapid and Accurate Identification of Four Waterfowl Parvoviruses (cGPV, MDPV, MDGPV, and SBDSV).Transboundary and emerging diseases · 2025Article
- One-Step Multiplex Real-Time Fluorescent Quantitative Reverse Transcription PCR for Simultaneous Detection of Four Waterfowl Viruses.Microorganisms · 2024Article
- Specific detection of duck adeno-associated virus using a TaqMan-based real-time PCR assay.Frontiers in veterinary science · 2024Article
- Development and application of quadruplex real time quantitative PCR method for differentiation of Muscovy duck parvovirus, Goose parvovirus, Duck circovirus, and Duck adenovirus 3.Frontiers in cellular and infection microbiology · 2024Article
- Emergence and genomic analysis of a novel ostrich-origin GPV-related parvovirus in China.Poultry science · 2022Article
- Identification and Pathogenicity Analysis of the Pathogen Causing Spotted Spleen in Muscovy Duck.Frontiers in veterinary science · 2022Article
- Advances in Research on Genetic Relationships of Waterfowl Parvoviruses.Journal of veterinary research · 2021Article
- Establishment of hydrolysis probe system real-time PCR assay for rapid detection of canine circovirus.3 Biotech · 2021Article
- Establishment of a duplex SYBR green I-based real-time polymerase chain reaction assay for the rapid detection of canine circovirus and canine astrovirus.Molecular and cellular probes · 2020Article
- Detection of Novel Goose Parvovirus Disease Associated with Short Beak and Dwarfism Syndrome in Commercial Ducks.Animals : an open access journal from MDPI · 2020Article
- A TaqMan-based real-time PCR assay for specific detection of novel duck reovirus in China.BMC veterinary research · 2020Article
- Specific detection and differentiation of classic goose parvovirus and novel goose parvovirus by TaqMan real-time PCR assay, coupled with host specificity.BMC veterinary research · 2019Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
backgroundMuscovy duck parvovirus (MDPV) causes high mortality and morbidity in Muscovy ducks, with the pathogenesis of the virus still unknown in many respects. Specific MDPV detection is often rife with false positive results because of high identity at the genomic nucleotide level and antigenic similarity with goose parvovirus (GPV). The objective of this study was to develop a sensitive, highly specific, and repeatable TaqMan-based real-time PCR (qPCR) assay for facilitating the molecular detection of MDPV.
resultsThe specific primers and probe were designed based on the conserved regions within MDPVs, but there was a variation in GPVs of the nonstructural (NS) genes after genetic comparison. After the optimization of qPCR conditions, the detection limit of this qPCR assay was 29.7 copies/μl. The assay was highly specific for the detection of MDPV, and no cross-reactivity was observed with other non-targeted duck-derived pathogens. Intra- and inter-assay variability was less than 2.21%, means a high degree of repeatability. The diagnostic applicability of the qPCR assay was proven that MDPV-positive can be found in cloacal swabs samples, Muscovy duck embryos and newly hatched Muscovy ducklings.
conclusionsOur data provided incidents that MDPV could be possible vertically transmitted from breeder Muscovy ducks to Muscovy ducklings. The developed qPCR assay in the study could be a reliable and specific tool for epidemiological surveillance and pathogenesis studies of MDPV.
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