ArticleMicrobial cell factories2018
Magnetization of active inclusion bodies: comparison with centrifugation in repetitive biotransformations.
Article in Microbial cell factories, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Carrier-Free Enzyme Immobilization for High-Density Catalytic Architectures in Flow Biocatalysis.JACS Au · 2026Review
- Active Inclusion Bodies in the Multienzymatic Synthesis of UDP-N-acetylglucosamine.International journal of molecular sciences · 2025Article
- Magnetizing Biotech-Advances in (In Vivo) Magnetic Enzyme Immobilization.Engineering in life sciences · 2025Review
- Physiologically Aggregated LacZ Applied in Trehalose Galactosylation in a Recycled Batch Mode.Life (Basel, Switzerland) · 2023Article
- Coiled-Coil Based Inclusion Bodies and Their Potential Applications.Frontiers in bioengineering and biotechnology · 2021Review
- Catalytically-active inclusion bodies for biotechnology-general concepts, optimization, and application.Applied microbiology and biotechnology · 2020Review
- Active tyrosine phenol-lyase aggregates induced by terminally attached functional peptides in Escherichia coli.Journal of industrial microbiology & biotechnology · 2020Article
- Leloir Glycosyltransferases in Applied Biocatalysis: A Multidisciplinary Approach.International journal of molecular sciences · 2019Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
backgroundPhysiological aggregation of a recombinant enzyme into enzymatically active inclusion bodies could be an excellent strategy to obtain immobilized enzymes for industrial biotransformation processes. However, it is not convenient to recycle "gelatinous masses" of protein inclusion bodies from one reaction cycle to another, as high centrifugation forces are needed in large volumes. The magnetization of inclusion bodies is a smart solution for large-scale applications, enabling an easier separation process using a magnetic field.
resultsMagnetically modified inclusion bodies of UDP-glucose pyrophosphorylase were recycled 50 times, in comparison, inclusion bodies of the same enzyme were inactivated during ten reaction cycles if they were recycled by centrifugation. Inclusion bodies of sialic acid aldolase also showed good performance and operational stability after the magnetization procedure.
conclusionsIt is demonstrated here that inclusion bodies can be easily magnetically modified by magnetic iron oxide particles prepared by microwave-assisted synthesis from ferrous sulphate. The magnetic particles stabilize the repetitive use of the inclusion bodies .
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