ArticleBMC genomics2018
CRISPR/Cas9-mediated gene knockin in the hydroid Hydractinia symbiolongicarpus.
Article in BMC genomics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 64 citations in OpenAlex.
- Problems With GO Enrichment as an Endpoint for Ecological Omics Interpretation.Molecular ecology · 2026Article
- Efficient genome editing using CRISPR-Cas9 in reef-building corals.Nature protocols · 2026Review
- Article
- Investigating the evolution and features of regeneration using cnidarians.Integrative and comparative biology · 2025Review
- Microinjection, gene knockdown, and CRISPR-mediated gene knock-in in the hard coral, Astrangia poculata.EvoDevo · 2025Article
- The Hydractinia cell atlas reveals cellular and molecular principles of cnidarian coloniality.Nature communications · 2025Article
- A positive feedback loop between germ cells and gonads induces and maintains sexual reproduction in a cnidarian.Science advances · 2025Article
- Neuronal and non-neuronal functions of the synaptic cell adhesion molecule neurexin in Nematostella vectensis.Nature communications · 2024Article
- Large-scale deorphanization ofeLife · 2024Article
- Topological structures and syntenic conservation in sea anemone genomes.Nature communications · 2023Article
- Future research directions of the model marine tubeworm Hydroides elegans and synthesis of developmental staging of the complete life cycle.Developmental dynamics : an official publication of the American Association of Anatomists · 2023Article
- Coevolution of the Tlx homeobox gene with medusa development (Cnidaria: Medusozoa).Communications biology · 2023Article
- Parallels and contrasts between the cnidarian and bilaterian maternal-to-zygotic transition are revealed in Hydractinia embryos.PLoS genetics · 2023Article
- Chromosome-level genome assembly of Hydractinia symbiolongicarpus.G3 (Bethesda, Md.) · 2023Article
- Parallels and contrasts between the cnidarian and bilaterian maternal-to-zygotic transition are revealed inbioRxiv : the preprint server for biology · 2023Article
- XY sex determination in a cnidarian.BMC biology · 2023Article
- Endogenous tagging of multiple cellular components in the sea anemoneProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Multiple Alr genes exhibit allorecognition-associated variation in the colonial cnidarian Hydractinia.Immunogenetics · 2022Article
- A family of unusual immunoglobulin superfamily genes in an invertebrate histocompatibility complex.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Cryopreservation ofAnimals : an open access journal from MDPI · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundHydractinia symbiolongicarpus, a colonial cnidarian, is a tractable model system for many cnidarian-specific and general biological questions. Until recently, tests of gene function in Hydractinia have relied on laborious forward genetic approaches, randomly integrated transgenes, or transient knockdown of mRNAs.
resultsHere, we report the use of CRISPR/Cas9 genome editing to generate targeted genomic insertions in H. symbiolonigcarpus. We used CRISPR/Cas9 to promote homologous recombination of two fluorescent reporters, eGFP and tdTomato, into the Eukaryotic elongation factor 1 alpha (Eef1a) locus. We demonstrate that the transgenes are expressed ubiquitously and are stable over two generations of breeding. We further demonstrate that CRISPR/Cas9 genome editing can be used to mark endogenous proteins with FLAG or StrepII-FLAG affinity tags to enable in vivo and ex vivo protein studies.
conclusionsThis is the first account of CRISPR/Cas9 mediated knockins in Hydractinia and the first example of the germline transmission of a CRISPR/Cas9 inserted transgene in a cnidarian. The ability to precisely insert exogenous DNA into the Hydractinia genome will enable sophisticated genetic studies and further development of functional genomics tools in this understudied cnidarian model.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.