ArticleThe Journal of veterinary medical science2018
Identification of reference genes for quantitative PCR analyses in developing mouse gonads.
Article in The Journal of veterinary medical science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
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- Ramadan fasting model modulates biomarkers of longevity and metabolism in male obese and non-obese rats.Scientific reports · 2024Article
- Characterization of dUTPase expression in mouse postnatal development and adult neurogenesis.Scientific reports · 2024Article
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- Optimal reference genes for RNA tissue analysis in small animal models of hemorrhagic fever viruses.Scientific reports · 2023Article
- Selection of species specific panel of reference genes in peripheral blood mononuclear cells of native livestock species adapted to trans-Himalayan region of Leh-Ladakh.Scientific reports · 2022Article
- A Short Promoter Region Containing Conserved Regulatory Motifs Is Required for Steroidogenic Acute Regulatory Protein (International journal of molecular sciences · 2022Article
- Evaluation of TILI-2 as an Anti-Tyrosinase, Anti-Oxidative Agent and Its Role in Preventing Melanogenesis Using a Proteomics Approach.Molecules (Basel, Switzerland) · 2022Article
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- Establishment of an organ culture system to induce Sertoli cell differentiation from undifferentiated mouse gonads.The Journal of veterinary medical science · 2020Article
- Observational
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Authors and funding
9 authors.
Funding
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Abstract
Stable reference genes are important for gene expression analyses such as quantitative PCR. The stability of 15 candidate reference genes that can be used to developing mouse gonads was thoroughly verified using combinations of multiple algorithms. The expression of these genes fluctuated greatly depending on the analysis period and/or gender. Peptidylprolyl isomerase A (Ppia) and polymerase (RNA) II (DNA directed) polypeptide A (Polr2a) were the reference genes that were used stably for a wide analysis period in developing mouse gonads. Furthermore, the stable reference genes corresponding to the analysis period and/or gender were ranked. These results are useful for the selection of the optimal reference gene required for high-precision measurements.
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