Evidence map›Paper›PMID 30175467›Full record

ArticleJournal of clinical laboratory analysis2019

New method to preserve the original proportion and integrity of urinary cell-free DNA.

Pei Li, Jun Ning, Xipeng Luo, Hongli Du, Qing Zhang, Ganlin Zhou, Qiu Du, Zhenyu Ou, Long Wang, Yu Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical laboratory analysis, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

  1. Review
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  10. Review
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  13. Technical and Methodological Aspects of Cell-Free Nucleic Acids Analyzes.International journal of molecular sciences · 2020
    Review
  14. Article
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Pei LiXiangYa Hospital of Central South University, Changsha, Hunan, China.
Jun NingXiangYa Hospital of Central South University, Changsha, Hunan, China.
Xipeng LuoHunan UPSBio, Inc., Hunan University National Science Park, Changsha, Hunan, China.
Hongli DuSchool of Bioscience and Bioengineering, South China University of Technology, Guangzhou, Guangdong, China.
Qing ZhangHunan UPSBio, Inc., Hunan University National Science Park, Changsha, Hunan, China.
Ganlin ZhouHunan UPSBio, Inc., Hunan University National Science Park, Changsha, Hunan, China.
Qiu DuXiangYa Hospital of Central South University, Changsha, Hunan, China.
Zhenyu OuXiangYa Hospital of Central South University, Changsha, Hunan, China.
Long WangXiangYa Hospital of Central South University, Changsha, Hunan, China.ORCID http://orcid.org/0000-0003-4583-2428
Yu WangHunan UPSBio, Inc., Hunan University National Science Park, Changsha, Hunan, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDue to high nuclease activity and complex contents in urine, urinary cell-free DNA (ucfDNA) was prone to degrade. So, we developed standardized urine collection tube (UCT) to prevent ucfDNA degradation and simultaneously maintain urinary cells in their original form during the sample collection process, ensuring stabilization of the original proportion and integrity of ucfDNA.

methodsUrine samples were collected from bladder cancer patients and divided into 10-mL normal tubes and 10-mL UCTs, respectively, and kept at ambient temperature. Urine supernatant was separated by centrifuging, and ucfDNA was extracted. Then ucfDNA was quantified by quantitative real-time polymerase chain reaction. UcfDNA fragments distribution was analyzed by Agilent 2200, and the frequency of specific mutations of urinary system disease was detected by next-generation sequencing method.

resultsUrine collected into UCTs showed no statistically significant changes in their original proportion and integrity of ucfDNA up to 7 days at ambient temperature and also ucfDNA fragments were maintained well. Conversely, urine collected into normal tubes was observed an obviously decline in their original proportion of ucfDNA and ucfDNA fragments changed greatly. The △% of allele fraction (AF) for specific genes of ucfDNA from UCTs was lower than from normal tubes by 3.7-fold.

conclusionUsing UCTs, they can maximally keep the original proportion and integrity of ucfDNA and stabilize urinary cells and minimize the background noise caused by urinary cellular DNA releasing, it will be help to open the door of next-generation noninvasive liquid biopsy applications utilizing urine.

Indexed as

Biomarkers, TumorCell-Free Nucleic AcidsHumansLiquid BiopsyReproducibility of ResultsSpecimen HandlingUrinary Bladder NeoplasmsBiomarkers, TumorCell-Free Nucleic Acidsliquid biopsynoninvasivepreservationurinary cell-free DNAurine

Identifiers

PMID30175467
PMCPMC6818579

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.