ArticlePlant diversity2018
A comparison of different methods for preserving plant molecular materials and the effect of degraded DNA on ddRAD sequencing.
Article in Plant diversity, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Nutritional supplement containing a nuclear fraction of bovine thymus gland increases the circulating levels of spermidine.PloS one · 2025Article
- From common gardens to candidate genes: exploring local adaptation to climate in red spruce.The New phytologist · 2023Article
- A Phylogenetic and Morphological Evolution Study ofPlants (Basel, Switzerland) · 2023Article
- Quality of DNA extracted from freshwater fish scales and mucus and its application in genetic diversity studies ofBiology methods & protocols · 2023Article
- DNA degradation in fish: Practical solutions and guidelines to improve DNA preservation for genomic research.Ecology and evolution · 2020Article
Corrections and comments
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Authors and funding
5 authors.
Funding
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Abstract
Obtaining high-quality plant materials for experiments is challenging for many research projects. Therefore, it is of special importance to determine the best method for preserving biological macromolecules like DNA, which degrade over time. Although some research has demonstrated that DNA degradation has little effect on traditional molecular markers, the effects of DNA degradation on ddRAD-seq, a popular reduced-representation sequencing technology, have not been adequately investigated. In this study, we first chose six woody bamboo species (Bambusoideae, Poaceae) to explore appropriate methods for preserving molecular materials with two DNA extraction approaches. Then we sequenced twenty-one bamboos and examined the effects of DNA quality on data generation using the ddRAD-seq technique (
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