ArticleVeterinary microbiology2018
Detection of foot-and-mouth disease virus in milk samples by real-time reverse transcription polymerase chain reaction: Optimisation and evaluation of a high-throughput screening method with potential for disease surveillance.
Article in Veterinary microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- Article
- Qualitative Risk Assessment of Foot-and-Mouth Disease Virus Introduction and Transmission to Dairy Farms via Raw Milk Transportation in Thailand: A Scenario-Based Approach.Veterinary sciences · 2025Article
- Emergency response for recently isolated Foot and Mouth Disease virus type A Africa in Egypt 2022.Scientific reports · 2025Article
- Detection of foot-and-mouth disease virus in raw milk in Menofia Governorate and its effect on reproductive hormones and physiochemical properties of milk.Veterinary world · 2022Article
- Development of a transboundary model of livestock disease in Europe.Transboundary and emerging diseases · 2022Article
- After nasopharyngeal infection, foot-and-mouth disease virus serotype A RNA is shed in bovine milk without associated mastitis.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2021Article
- Scientific Opinion on the assessment of the control measures for category A diseases of Animal Health Law: Foot and Mouth Disease.EFSA journal. European Food Safety Authority · 2021Article
- Utilizing milk from pooling facilities as a novel approach for foot-and-mouth disease surveillance.Transboundary and emerging diseases · 2020Article
- Advances in the Diagnosis of Foot-and-Mouth Disease.Frontiers in veterinary science · 2020Review
- Foot-and-Mouth Disease Surveillance Using Pooled Milk on a Large-Scale Dairy Farm in an Endemic Setting.Frontiers in veterinary science · 2020Article
- Opportunities for enhanced surveillance of foot-and-mouth disease in endemic settings using milk samples.Transboundary and emerging diseases · 2019Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study aimed to evaluate the utility of milk as a non-invasive sample type for the surveillance of foot-and-mouth disease (FMD), a highly contagious viral disease of cloven-hooved animals. Four milking Jersey cows were infected via direct-contact with two non-milking Jersey cows that had been previously inoculated with FMD virus (FMDV: isolate O/UKG/34/2001). Milk and blood were collected throughout the course of infection to compare two high-throughput real-time reverse transcription polymerase chain reaction (rRT-PCR) protocols with different RT-PCR chemistries. Using both methods, FMDV was detected in milk by rRT-PCR one to two days before the presentation of characteristic foot lesions, similar to detection by virus isolation. Furthermore, rRT-PCR detection from milk was extended, up to 28 days post contact (dpc), compared to detection by virus isolation (up to 14 dpc). Additionally, the detection of FMDV in milk by rRT-PCR was possible for 18 days longer than detection by the same method in serum samples. FMDV was also detected with both rRT-PCR methods in milk samples collected during the UK 2007 outbreak. Dilution studies were undertaken using milk from the field and experimentally-infected animals, where for one sample it was possible to detect FMDV at 10
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