ArticleMicrobial cell factories2018
Engineering the oleaginous yeast Yarrowia lipolytica to produce the aroma compound β-ionone.
Article in Microbial cell factories, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 107 citations in OpenAlex.
- Engineering Modification for the Efficient Synthesis of β-ionone by Yarrowia lipolytica.Biotechnology journal · 2026Article
- Artificial Intelligence for Microbial Isolation and Cultivation: Progress and Challenges.Microorganisms · 2026Review
- Division of labour enables Yarrowia lipolytica synthetic consortia to achieve higher growth rates from starch.Microbial cell factories · 2026Review
- Metabolic engineering ofFrontiers in microbiology · 2026Article
- Identification, Cloning, and Functional Characterization of Carotenoid Cleavage Dioxygenase (CCD) fromBiology · 2025Article
- Synthesis of β-ionone from xylose and lignocellulosic hydrolysate in genetically engineered oleaginous yeast Yarrowia lipolytica.Biotechnology letters · 2024Article
- Introducing CCD1 into isolatedFrontiers in bioengineering and biotechnology · 2024Article
- Progress in the Metabolic Engineering ofBiodesign research · 2024Review
- Characterization of Volatile Organic Compounds and Aroma Sensory Properties in Yunnan Cigar.Journal of analytical methods in chemistry · 2024Article
- Iterative gene integration mediated by 26S rDNA and non-homologous end joining for the efficient production of lycopene in Yarrowia lipolytica.Bioresources and bioprocessing · 2023Article
- Improving solubility and copy number of taxadiene synthase to enhance the titer of taxadiene inSynthetic and systems biotechnology · 2023Article
- Industrially Important Fungal Carotenoids: Advancements in Biotechnological Production and Extraction.Journal of fungi (Basel, Switzerland) · 2023Review
- Purification and biochemical characterization of a novel ene- reductase from Kazachstania exigua HSC6 for dihydro-β-ionone from β-ionone.Biotechnology letters · 2023Article
- Photobiological production of high-value pigments via compartmentalized co-cultures using Ca-alginate hydrogels.Scientific reports · 2022Article
- Genome-wide CRISPR-Cas9 screen reveals a persistent null-hyphal phenotype that maintains high carotenoid production in Yarrowia lipolytica.Biotechnology and bioengineering · 2022Article
- Engineering ofMetabolic engineering communications · 2022Review
- Characteristics of NtCCD1-3 from tobacco, and protein engineering of the CCD1 to enhanceFrontiers in microbiology · 2022Article
- Efficient production of the β-ionone aroma compound from organic waste hydrolysates using an engineeredFrontiers in microbiology · 2022Article
- A Novel Cre/International journal of molecular sciences · 2021Article
- β-Ionone: Its Occurrence and Biological Function and Metabolic Engineering.Plants (Basel, Switzerland) · 2021Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundβ-Ionone is a fragrant terpenoid that generates a pleasant floral scent and is used in diverse applications as a cosmetic and flavoring ingredient. A growing consumer desire for natural products has increased the market demand for natural β-ionone. To date, chemical extraction from plants remains the main approach for commercial natural β-ionone production. Unfortunately, changing climate and geopolitical issues can cause instability in the β-ionone supply chain. Microbial fermentation using generally recognized as safe (GRAS) yeast offers an alternative method for producing natural β-ionone. Yarrowia lipolytica is an attractive host due to its oleaginous nature, established genetic tools, and large intercellular pool size of acetyl-CoA (the terpenoid backbone precursor).
resultsA push-pull strategy via genome engineering was applied to a Y. lipolytica PO1f derived strain. Heterologous and native genes in the mevalonate pathway were overexpressed to push production to the terpenoid backbone geranylgeranyl pyrophosphate, while the carB and biofunction carRP genes from Mucor circinelloides were introduced to pull flux towards β-carotene (i.e., ionone precursor). Medium tests combined with machine learning based data analysis and
conclusionsWe engineered a GRAS Y. lipolytica platform for sustainable and economical production of the natural aroma β-ionone. Although β-carotene could be produced at high titers by Y. lipolytica, the synthesis of β-ionone was relatively poor, possibly due to low CCD1 activity and non-specific CCD1 cleavage of β-carotene. In addition, both β-carotene and β-ionone strains showed decreased performances after successive sub-cultures. For industrial application, β-ionone fermentation efforts should focus on both CCD enzyme engineering and strain stability improvement.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.