ArticleMicrobiome2018
Detecting eukaryotic microbiota with single-cell sensitivity in human tissue.
Article in Microbiome, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
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- Maternal and placental microbiome and immune crosstalk in pregnancies with small-for-gestational-age fetuses - a pilot case-control study.Frontiers in cellular and infection microbiology · 2025Article
- Evolution of a Pathogenic Microbiome.Journal of clinical medicine · 2023Review
- A Repertoire of the Less Common Clinical Yeasts.Journal of fungi (Basel, Switzerland) · 2023Review
- Evaluating Current Molecular Techniques and Evidence in Assessing Microbiome in Placenta-Related Health and Disorders in Pregnancy.Biomolecules · 2023Review
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- Crucial nuances in understanding (mis)associations between the neonatal microbiome and Cesarean delivery.Trends in molecular medicine · 2022Review
- Comprehensive human amniotic fluid metagenomics supports the sterile womb hypothesis.Scientific reports · 2022Article
- Maternal and early life exposures and their potential to influence development of the microbiome.Genome medicine · 2022Review
- Fetal inheritance of chromosomally integrated human herpesvirus 6 predisposes the mother to pre-eclampsia.Nature microbiology · 2020Article
- Lack of Evidence for Microbiota in the Placental and Fetal Tissues of Rhesus Macaques.mSphere · 2020Article
- Article
- Article
- Does the human placenta delivered at term have a microbiota? Results of cultivation, quantitative real-time PCR, 16S rRNA gene sequencing, and metagenomics.American journal of obstetrics and gynecology · 2019Article
- Correction to: Detecting eukaryotic microbiota with single-cell sensitivity in human tissue.Microbiome · 2018Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundFetal growth restriction, pre-eclampsia, and pre-term birth are major adverse pregnancy outcomes. These complications are considerable contributors to fetal/maternal morbidity and mortality worldwide. A significant proportion of these cases are thought to be due to dysfunction of the placenta. However, the underlying mechanisms of placental dysfunction are unclear. The aim of the present study was to investigate whether adverse pregnancy outcomes are associated with evidence of placental eukaryotic infection.
resultsWe modified the 18S Illumina Amplicon Protocol of the Earth Microbiome Project and made it capable of detecting just a single spiked-in genome copy of Plasmodium falciparum, Saccharomyces cerevisiae, or Toxoplasma gondii among more than 70,000 human cells. Using this method, we were unable to detect eukaryotic pathogens in placental biopsies in instances of adverse pregnancy outcome (n = 199) or in healthy controls (n = 99).
conclusionsEukaryotic infection of the placenta is not an underlying cause of the aforementioned pregnancy complications. Possible clinical applications for this non-targeted, yet extremely sensitive, eukaryotic screening method are manifest.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.