Evidence map›Paper›PMID 30171813›Full record

ArticleTransfusion2018

A comparison of different methods of red blood cell leukoreduction and additive solutions on the accumulation of neutrophil-priming activity during storage.

Michele M Loi, Marguerite Kelher, Monika Dzieciatkowska, Kirk C Hansen, Anirban Banerjee, F Bernadette West, Crystal Stanley, Matthew Briel, Christopher C Silliman

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Transfusion, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Pooled it
  2. Pooled it
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Michele M LoiDepartment of Research Laboratory, University of Colorado Denver, Aurora, Colorado.
Marguerite KelherDepartment of Research Laboratory, University of Colorado Denver, Aurora, Colorado.
Monika DzieciatkowskaDepartment of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, Colorado.
Kirk C HansenDepartment of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, Colorado.
Anirban BanerjeeDepartment of Surgery, University of Colorado Denver, Aurora, Colorado.
F Bernadette WestConnecticut, Mid-Atlantic, and Appalachian Regions, American Red Cross, Hartford, Connecticut.
Crystal StanleyManufacturing, Bonfils Blood Center, Denver, Colorado.
Matthew BrielManufacturing, Bonfils Blood Center, Denver, Colorado.
Christopher C SillimanDepartment of Research Laboratory, University of Colorado Denver, Aurora, Colorado.

Funding

Trauma Reprograms CellsP50GM049222 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI BANERJEE, ANIRBAN · 1993 to 2017
$26.1M
Mechanisms of Trauma-Induced CoagulopathyT32GM008315 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI Mitchell Cohen, ELIZABETH J. KOVACS · 1991 to 2026
$7.1M
POSTGRADUATE TRAINING IN PEDIATRIC ONCOLOGYT32CA082086 · NCI · UNIVERSITY OF COLORADO DENVER · PI GORE, LIA · 2000 to 2015
$4.1M
Lyso-PCs activate PMNs and endothelium via PKCR01HL059355 · NHLBI · UNIVERSITY OF COLORADO DENVER · PI SILLIMAN, CHRISTOPHER C. · 1999 to 2006
$1.7M
NCI NIH HHS T32 CA082086NHLBI NIH HHS R01 HL059355NIGMS NIH HHS P50 GM049222NIGMS NIH HHS T32 GM008315
6 · The paper itself

Abstract

backgroundThree methods of leukoreduction (LR) are used worldwide: filtration, buffy coat removal (BCR), and a combination of the previous two methods. Additionally, there are a number of additive solutions (ASs) used to preserve red blood cell (RBC) function throughout storage. During RBC storage, proinflammatory activity accumulates; thus, we hypothesize that both the method of LR and the AS affect the accumulation of proinflammatory activity. STUDY DESIGN AND

methodsTen units of whole blood were drawn from healthy donors, the RBC units were isolated, divided in half by weight, and leukoreduced by: 1) BCR, 2) filtration, or 3) BCR and filtration (combination-LR); stored in bags containing AS-3 per AABB criteria; and sampled weekly. The supernatants were isolated and frozen (-80°C). RBC units drawn from healthy donors into AS-1-, AS-3-, or AS-5-containing bags were also stored and sampled weekly, and the supernatants were isolated and frozen. The supernatants were assayed for neutrophil (PMN)-priming activity and underwent proteomic analyses.

resultsFiltration and combination LR decreased priming activity accumulation versus buffy coat LR, although the accumulation of priming activity was not different during storage. Combination LR increased hemolysis versus filtration via proteomic analysis. Priming activity from AS-3 units was significant later in storage versus AS-1- or AS-5-stored units.

conclusionsAlthough both filtration and combination LR decrease the accumulation of proinflammatory activity versus buffy coat LR, combination LR is not more advantageous over filtration, has increased costs, and may cause increased hemolysis. In addition, AS-3 decreases the early accumulation of PMN-priming activity during storage versus AS-1 or AS-5.

Indexed as

Leukocyte Reduction ProceduresNeutrophil ActivationBlood Buffy CoatBlood PreservationErythrocytesFiltrationHumansInflammationMethodsSolutionsSolutions

Identifiers

PMID30171813
PMCPMC6131068

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.