Evidence map›Paper›PMID 30171517›Full record

ArticleMolecular biotechnology2018

Identification of Potential Molecular Determinants of Murine Embryonic Stem Cell Differentiation by a Transposon-Based Approach.

Yan Wang, Tingjun Lei, Qian Dai, Ping Ding, Tong Qiu, Yin Fang

Abstract read
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In one paragraph

Article in Molecular biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Yan WangDepartment of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.
Tingjun LeiDepartment of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.
Qian DaiDepartment of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.
Ping DingSichuan Cunde Therapeutics, Chengdu, 610093, China.
Tong QiuDepartment of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu, 610041, Sichuan, China. qiutong08@hotmail.com.
Yin FangDepartment of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu, 610041, Sichuan, China. myt_kkxx2008@126.com.

Funding

Department of Science and Technology of Sichuan Province 2016JQ0029National Natural Science Foundation of China 81671115
6 · The paper itself

Abstract

Embryonic stem cells (ESCs) are self-renewing pluripotent cells, capable of differentiating into all somatic cell types. The molecular control of self-renewal is relatively well-characterized, whereas how ESCs exit pluripotent state to differentiate is poorly understood. Here we identify two genes are required for differentiation and dozens of intergenic regions that potentially regulate ESC differentiation. We used PiggyBac (PB) transposon-based approach to randomly mutate the genome of ESCs, and generated hundreds of clones that resisted differentiation signals. Each clone was sequenced to determine genomic regions mutated by PB insertion. Intriguingly, many mutations were localized among intergenic regions and we identified two genes are required for differentiation. This study should facilitate further exploration of novel molecular determinants of embryonic stem cell differentiation.

Indexed as

DNA Transposable ElementsAnimalsCell DifferentiationCell ProliferationMAP Kinase Signaling SystemMiceMouse Embryonic Stem CellsMutagenesis, InsertionalMutationSequence Analysis, DNADNA Transposable ElementsCell differentiationEmbryonic stem cellMitogen-activated protein kinase (MAPK)PiggyBac transposonPluripotencyStromal interaction molecule 1 (STIM1)

Identifiers

PMID30171517

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.