ArticleAMB Express2018
Optimization of the culture condition of Bacillus mucilaginous using Agaricus bisporus industrial wastewater by Plackett-Burman combined with Box-Behnken response surface method.
Article in AMB Express, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed.
- Optimization of the Extraction of Total Alkaloids From Lotus Leaves by Acidic Ethanol and Its Purification by Macroporous Resins.Biomedical chromatography : BMC · 2026Article
- Optimization of triterpenoids biosynthesis in Athelia termitophila as a source of natural products.AMB Express · 2025Article
- Behavior and Mechanisms of Antimony Precipitation from Wastewater by Sulfate-Reducing BacteriaToxics · 2024Article
- Purification and characterization of the low molecular weight xylanase from Bacillus cereus L-1.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- A design of experiments screen reveals that Clostridium novyi-NT spore germinant sensing is stereoflexible for valine and its analogs.Communications biology · 2023Article
- Influence of medium modifications (optimization) on high nematicidal activity of the fermentation broth ofFrontiers in bioengineering and biotechnology · 2023Article
- Optimization of bacterial sporulation using economic nutrient for self-healing concrete.Journal of microbiology (Seoul, Korea) · 2020Article
- Optimization of Culture Conditions for Amoxicillin Degrading Bacteria Screened from Pig Manure.International journal of environmental research and public health · 2020Article
- Article
- Hydrolytic denitrification and decynidation of acrylonitrile in wastewater with Arthrobacter nitroguajacolicus ZJUTB06-99.AMB Express · 2018Article
- Optimization of Cultural Conditions forBioMed research international · 2018Article
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5 authors.
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Abstract
In the present study, conditions for Bacillus mucilaginous fermentation using Agaricus bisporus wastewater as culture medium were optimized. We analyzed the total number of living B. mucilaginous in the fermentation broth using multispectral imaging flow cytometry. Single-factor experiments were carried out, where a Plackett-Burman design was used to screen out three factors from the original six factors of processing wastewater solubility, initial pH, inoculum size, liquid volume, culture temperature, and rotation speed that affected the total number of viable B. mucilaginous. The Box-Behnken response surface method was used to optimize interactions between the three main factors and predict optimal fermentation conditions. Factors significantly affecting the total number of viable B. mucilaginous, including shaking speed, culturing temperature, and initial pH, were investigated. The optimum conditions for B. mucilaginous fermentation in A. bisporus wastewater were a rotational speed of 195 rpm, culture temperature of 29 °C, initial pH of 6.5, solubility of 0.5%, 8% inoculation volume, and 90 mL liquid volume in a 250 mL flask, culture time of 48 h. Under these conditions, the concentration of total viable bacteria reached 2.16 ± 0.02 × 10
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