ArticleProceedings of the National Academy of Sciences of the United States of America2018
Machining protein microcrystals for structure determination by electron diffraction.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- Practical crystallography with a transmission electron microscope.Journal of microscopy · 2026Article
- Article
- Electrons and X-rays for diffraction and imaging.Acta crystallographica. Section D, Structural biology · 2026Review
- Single-cell structural biology with intracellular electron crystallography.Nature communications · 2026Article
- Advances in Crystallography and Cryo-Electron Microscopy for the Study of Membrane Proteins.Advances in experimental medicine and biology · 2026Review
- Strategies for mitigating radiation damage and improving data completeness in 3D electron diffraction of protein crystals.Acta crystallographica. Section D, Structural biology · 2026Article
- Review
- Fast event-based electron counting for small-molecule structure determination by MicroED.Acta crystallographica. Section C, Structural chemistry · 2025Article
- VMXm - A sub-micron focus macromolecular crystallography beamline at Diamond Light Source.Journal of synchrotron radiation · 2024Article
- Doses for X-ray and electron diffraction: New features in RADDOSE-3D including intensity decay models.Protein science : a publication of the Protein Society · 2024Article
- Applying 3D ED/MicroED workflows toward the next frontiers.Acta crystallographica. Section C, Structural chemistry · 2024Article
- Focused ion beam milling and MicroED structure determination of metal-organic framework crystals.Ultramicroscopy · 2024Article
- Advances and applications of microcrystal electron diffraction (MicroED).Current opinion in structural biology · 2024Review
- Structure determination of a DNA crystal by MicroED.Structure (London, England : 1993) · 2023Article
- Polymorphic nanobody crystals as long-acting intravitreal therapy for wet age-related macular degeneration.Bioengineering & translational medicine · 2023Article
- Article
- A robust approach for MicroED sample preparation of lipidic cubic phase embedded membrane protein crystals.Nature communications · 2023Article
- Electron Diffraction of 3D Molecular Crystals.Chemical reviews · 2022Review
- Better, Faster, Cheaper: Recent Advances in Cryo-Electron Microscopy.Annual review of biochemistry · 2022Review
- Ab initio phasing macromolecular structures using electron-counted MicroED data.Nature methods · 2022Article
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Authors and funding
10 authors.
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Abstract
We demonstrate that ion-beam milling of frozen, hydrated protein crystals to thin lamella preserves the crystal lattice to near-atomic resolution. This provides a vehicle for protein structure determination, bridging the crystal size gap between the nanometer scale of conventional electron diffraction and micron scale of synchrotron microfocus beamlines. The demonstration that atomic information can be retained suggests that milling could provide such detail on sections cut from vitrified cells.
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