Evidence map›Paper›PMID 30170240›Full record

ArticleVirology2018

Production and characterization of a novel HPV anti-L2 monoclonal antibody panel.

S M Bywaters, S A Brendle, J Biryukov, J W Wang, J Walston, J Milici, R B Roden, C Meyers, N D Christensen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

S M BywatersDepartment of Microbiology and Immunology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA. Electronic address: smb581@psu.edu.
S A BrendleJake Gittlen Laboratories for Cancer Research, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA. Electronic address: sab40@psu.edu.
J BiryukovDepartment of Microbiology and Immunology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA. Electronic address: jlh511@psu.edu.
J W WangDepartment of Pathology, Johns Hopkins University, Baltimore, MD, USA. Electronic address: jwang@pathovax.com.
J WalstonDepartment of Microbiology and Immunology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA. Electronic address: jpw5487@psu.edu.
J MiliciDepartment of Microbiology and Immunology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA. Electronic address: jem36@psu.edu.
R B RodenDepartment of Pathology, Johns Hopkins University, Baltimore, MD, USA. Electronic address: roden@jhmi.edu.
C MeyersDepartment of Microbiology and Immunology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA. Electronic address: cmm10@psu.edu.
N D ChristensenJake Gittlen Laboratories for Cancer Research, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA. Electronic address: ndc1@psu.edu.

Funding

Treatment of HIV- and HIV+ Patients with HPV16+ CIN2/3 Using pNGLV4a-hCRTE6E7L2 DNA vaccine administered intramuscularly via electroporationP50CA098252 · NCI · JOHNS HOPKINS UNIVERSITY · PI TZYY-CHOOU WU · 2003 to 2026
$53.5M
VIRUSES AND CANCERT32CA060395 · NCI · PENNSYLVANIA STATE UNIV HERSHEY MED CTR · PI MEYERS, CRAIG M · 1994 to 2021
$4.3M
Mechanisms of Papillomavirus NeutralizationR01CA118790 · NCI · JOHNS HOPKINS UNIVERSITY · PI RODEN, RICHARD BRUCE · 2007 to 2018
$2.9M
NCI NIH HHS P50 CA098252NCI NIH HHS R01 CA118790NCI NIH HHS T32 CA060395
6 · The paper itself

Abstract

The major capsid protein of HPV, L1, assembles into pentamers that form a T = 7 icosahedral particle, but the location of the co-assembled minor capsid protein, L2, remains controversial. Several researchers have developed useful monoclonal antibodies targeting L2, but most react with linear epitopes toward the N-terminus. As a means to better define the virus capsid and better assess the localization and exposure of L2 epitopes in the context of assembled HPV, we have developed a panel of 30 monoclonal antibodies (mAbs) which target the N-terminus of L2 amino acids 11-200, previously defined as a broadly protective immunogen. Select mAbs were processed with enzymes and anti-L2 Fabs were generated. These new mAb/Fab probes will be beneficial in future studies to unravel the placement of L2 and to help better define the role of L2 in the HPV lifecycle and the nature of the broadly protective epitopes.

Indexed as

AlphapapillomavirusAnimalsAntibodies, MonoclonalAntibodies, ViralCapsid ProteinsCross ReactionsEpitopesHumansMiceMice, Inbred BALB COncogene Proteins, ViralPapillomavirus InfectionsVirionAntibodies, MonoclonalAntibodies, ViralCapsid ProteinsEpitopesL2 protein, Human papillomavirus type 16Oncogene Proteins, ViralAntibodyEpitopeFabHPVL2mAbMinor capsid protein

Identifiers

PMID30170240
PMCPMC6309409

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.