ArticleJournal of experimental botany2018
A BAHD neofunctionalization promotes tetrahydroxycinnamoyl spermine accumulation in the pollen coat of the Asteraceae family.
Article in Journal of experimental botany, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 15 citations in OpenAlex.
- Integrated metabolomics and antioxidant activity assessment of Sphagneticola trilobata (L.) Pruski.Scientific reports · 2026Article
- Natural Variations in the Activity of Hydroxycinnamoyl Transferases Promote Accumulation of Metabolites Conferring Rice Resistance.Plant biotechnology journal · 2026Article
- Learning from nature: phytochemical strategies to protect against UV-B damage.Nature communications · 2025Review
- Hydroxycinnamoyl-coenzyme A: tetrahydroxyhexanedioate hydroxycinnamoyl transferase (HHHT) fromPeerJ · 2025Article
- Discovering a mitochondrion-localized BAHD acyltransferase involved in calystegine biosynthesis and engineering the production of 3β-tigloyloxytropane.Nature communications · 2024Article
- Identification and profile of phenolamides with anthracnose resistance potential in tea (Horticulture research · 2023Article
- Article
- Consecutive action of two BAHD acyltransferases promotes tetracoumaroyl spermine accumulation in chicory.Plant physiology · 2022Article
- Transcriptomics and Metabolomics Analyses Reveal High Induction of the Phenolamide Pathway in Tomato Plants Attacked by the LeafminerMetabolites · 2022Article
- MeJA Elicitation of Chicory Hairy Roots Promotes Efficient Increase of 3,5-diCQA Accumulation, a Potent Antioxidant and Antibacterial Molecule.Antibiotics (Basel, Switzerland) · 2020Article
- Structural and Biochemical Insights Into Two BAHD Acyltransferases (Frontiers in plant science · 2020Article
- Efficient Genome Editing Using CRISPR/Cas9 Technology in Chicory.International journal of molecular sciences · 2019Article
- Identification and Functional Characterization of Genes Involved in the Biosynthesis of Caffeoylquinic Acids in Sunflower (Frontiers in plant science · 2019Article
- Unusual spermine-conjugated hydroxycinnamic acids on pollen: function and evolutionary advantage.Journal of experimental botany · 2018Article
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Authors and funding
12 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In eudicotyledons, accumulation of trihydroxycinnamoyl spermidine that is restricted to the pollen wall constitutes an evolutionary conserved trait. However, the role of this compound, which is synthetized by the BAHD enzyme spermidine hydroxycinnamoyl transferase (SHT), is still a matter of debate. Here, we show that this particular phenolamide is replaced by tetrahydroxycinnamoyl spermine in the pollen coat of the Asteraceae. Phylogenetic analyses combined with quantitative RT-PCR experiments allowed the identification of two homologous genes from Cichorium intybus (chicory) putatively involved in its metabolism. In vitro biochemical characterization of the two enzymes, named CiSHT1 and CiSHT2, confirmed the capability of recombinant proteins to synthesize spermine as well as spermidine derivatives. The wild-type metabolic phenotype was partially restored in an Arabidopsis sht mutant expressing CiSHT2. Strikingly, the transgenic plants also accumulated spermine derivatives that were absent in the wild-type. Overexpression of CiSHT2 in chicory hairy roots led to the accumulation of spermine derivatives, confirming its in vivo function. Complementary sequence analyses revealed the presence of an amino acid motif typical of the SHTs among the BAHD enzyme family. Our results highlight a recent neofunctionalization among the SHTs that has promoted the emergence of new phenolamides in the Asteraceae, which could potentially have contributed to the evolutionary success of this family.
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