ArticlePLoS pathogens2018
Critical role for a promoter discriminator in RpoS control of virulence in Edwardsiella piscicida.
Article in PLoS pathogens, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 53 citations in OpenAlex.
- Article
- ArgR-dependent bacterial resistance to host lipid droplets in Edwardsiella piscicida.Communications biology · 2025Article
- Cooperative mechanisms of LexA and HtpG in the regulation of virulence gene expression inCurrent research in microbial sciences · 2025Article
- Coevolution between marine Aeromonas and phages reveals temporal trade-off patterns of phage resistance and host population fitness.The ISME journal · 2023Article
- Design combinations of evolved phage and antibiotic for antibacterial guided by analyzing the phage resistance of poorly antimicrobial phage.Microbiology spectrum · 2023Article
- Identification of determinants for entering into a viable but nonculturable state in Vibrio alginolyticus by Tn-seq.Applied microbiology and biotechnology · 2023Article
- Versatile lifestyles ofVirulence · 2022Article
- Edwardsiella tarda TraT is an anti-complement factor and a cellular infection promoter.Communications biology · 2022Article
- Xenogeneic nucleoid-associated EnrR thwarts H-NS silencing of bacterial virulence with unique DNA binding.Nucleic acids research · 2022Article
- Transcriptomic and phenotype analysis revealed the role ofFrontiers in microbiology · 2022Article
- Article
- A Genome-Scale Antibiotic Screen in Serratia marcescens Identifies YdgH as a Conserved Modifier of Cephalosporin and Detergent Susceptibility.Antimicrobial agents and chemotherapy · 2021Article
- σFrontiers in microbiology · 2021Article
- Identification and Characterization of EvpQ, a Novel T6SS Effector Encoded on a Mobile Genetic Element inFrontiers in microbiology · 2021Article
- Article
- Review
- Article
- HutZ is required for biofilm formation and contributes to the pathogenicity of Edwardsiella piscicida.Veterinary research · 2019Article
- Genome-Wide Identification of Fitness Factors in Seawater forApplied and environmental microbiology · 2019Article
- Thioredoxin H (TrxH) contributes to adversity adaptation and pathogenicity of Edwardsiella piscicida.Veterinary research · 2019Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 3 countries.
Funding
Abstract
Edwardsiella piscicida is a leading fish pathogen that causes significant economic loses in the aquaculture industry. The pathogen depends on type III and type VI secretion systems (T3/T6SS) for growth and virulence in fish and the expression of both systems is controlled by the EsrB transcription activator. Here, we performed a Tn-seq-based screen to uncover factors that govern esrB expression. Unexpectedly, we discovered that RpoS antagonizes esrB expression and thereby inhibits production of E. piscicida's T3/T6SS. Using in vitro transcription assays, we showed that RpoS can block RpoD-mediated transcription of esrB. ChIP-seq- and RNA-seq-based profiling, as well as mutational and biochemical analyses revealed that RpoS-repressed promoters contain a -6G in their respective discriminator sequences; moreover, this -6G proved critical for RpoS to inhibit esrB expression. Mutation of the RpoS R99 residue, an amino acid that molecular modeling predicts interacts with -6G in the esrB discriminator, abolished RpoS' capacity for repression. In a turbot model, an rpoS deletion mutant was attenuated early but not late in infection, whereas a mutant expressing RpoSR99A exhibited elevated fitness throughout the infection period. Collectively, these findings deepen our understanding of how RpoS can inhibit gene expression and demonstrate the temporal variation in the requirement for this sigma factor during infection.
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