ArticlePloS one2018
Hotspot mutations and ColE1 plasmids contribute to the fitness of Salmonella Heidelberg in poultry litter.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 46 citations in OpenAlex.
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- Prevalence, Risk Factors, and Human Health Implications of Salmonella enterica and Campylobacter spp. in Vermont Backyard Poultry.Zoonoses and public health · 2025Observational
- Phenotypic and Genomic Assessment of Antimicrobial Resistance and Virulence Factors Determinants inAnimals : an open access journal from MDPI · 2025Article
- Genomic insights into antimicrobial resistance and virulence ofFrontiers in microbiology · 2025Article
- Genotypic and phenotypic analysis ofFrontiers in microbiology · 2024Article
- Comprehensive Genomic Characterization of Antibiotic Resistance, Virulence, and Clonality in Salmonella Isolates from Wild Animals in Algeria.EcoHealth · 2023Article
- Dissemination of IncI plasmid encodingAntimicrobial agents and chemotherapy · 2023Article
- Genomic analyses of drug-resistant Salmonella enterica serovar Heidelberg strains isolated from meat and related sources between 2013 and 2017 in the south region of Brazil.Current genetics · 2023Article
- Assessment of plasmids for relating the 2020 Salmonella enterica serovar Newport onion outbreak to farms implicated by the outbreak investigation.BMC genomics · 2023Article
- A Familiar Outbreak of MonophasicPathogens (Basel, Switzerland) · 2022Article
- Combining analytical epidemiology and genomic surveillance to identify risk factors associated with the spread of antimicrobial resistance inMicrobial genomics · 2022Article
- A scoping review of factors potentially linked with antimicrobial-resistant bacteria from turkeys (iAM.AMR Project).Epidemiology and infection · 2022Article
- Litter Commensal Bacteria Can Limit the Horizontal Gene Transfer of Antimicrobial Resistance to Salmonella in Chickens.Applied and environmental microbiology · 2022Article
- Genomic and Evolutionary Analysis ofFrontiers in cellular and infection microbiology · 2022Article
- Salmonella Genomics in Public Health and Food Safety.EcoSal Plus · 2021Article
- Article
- Prevalence of IncFIB Plasmids Found amongPathogens (Basel, Switzerland) · 2021Article
- Genomic Analysis of Ciprofloxacin-ResistantFrontiers in microbiology · 2021Article
- Bacterial Detection and Recovery From Poultry Litter.Frontiers in microbiology · 2021Article
- Horizontal 'gene drives' harness indigenous bacteria for bioremediation.Scientific reports · 2020Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Salmonella enterica subsp. enterica serovar Heidelberg (S. Heidelberg) is a clinically-important serovar linked to food-borne illness, and commonly isolated from poultry. Investigations of a large, multistate outbreak in the USA in 2013 identified poultry litter (PL) as an important extra-intestinal environment that may have selected for specific S. Heidelberg strains. Poultry litter is a mixture of bedding materials and chicken excreta that contains chicken gastrointestinal (GI) bacteria, undigested feed, feathers, and other materials of chicken origin. In this study, we performed a series of controlled laboratory experiments which assessed the microevolution of two S. Heidelberg strains (SH-2813 and SH-116) in PL previously used to raise 3 flocks of broiler chickens. The strains are closely related at the chromosome level, differing from the reference genome by 109 and 89 single nucleotide polymorphisms/InDels, respectively. Whole genome sequencing was performed on 86 isolates recovered after 0, 1, 7 and 14 days of microevolution in PL. Only strains carrying an IncX1 (37kb), 2 ColE1 (4 and 6kb) and 1 ColpVC (2kb) plasmids survived more than 7 days in PL. Competition experiments showed that carriage of these plasmids was associated with increased fitness. This increased fitness was associated with an increased copy number of IncX1 and ColE1 plasmids. Further, all Col plasmid-bearing strains had hotspot mutations in 37 loci on the chromosome and in 3 loci on the IncX1 plasmid. Additionally, we observed a decrease in susceptibility to tobramycin, kanamycin, gentamicin, neomycin and fosfomycin for Col plasmid-bearing strains. Our study demonstrates how positive selection from poultry litter can change the evolutionary path of S. Heidelberg.
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