ArticleACS sensors2018
Influence of Fluorescent Protein Maturation on FRET Measurements in Living Cells.
Article in ACS sensors, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Chemical interactions in polyethylene glycol-induced condensates lead to an anomalous FRET response from a flexible linker-fluorescent protein crowding sensor.bioRxiv : the preprint server for biology · 2026Article
- Decoding mEos4b day-long maturation and engineering fast-maturing variants.Protein science : a publication of the Protein Society · 2025Article
- Two-point calibration protocol for the Förster Resonance Energy Transfer indicator Pyronic in neurons.Neurophotonics · 2025Article
- Liposome-EncapsulatedACS synthetic biology · 2025Article
- Simulation-based Reconstructed Diffusion unveils the effect of aging on protein diffusion in Escherichia coli.PLoS computational biology · 2023Article
- Monte Carlo samplers for efficient network inference.PLoS computational biology · 2023Article
- Article
- Gene expression model inference from snapshot RNA data using Bayesian non-parametrics.Nature computational science · 2023Article
- Correlating viscosity and molecular crowding with fluorescent nanobeads and molecular probes:Interface focus · 2022Article
- Quantification of Dark Protein Populations in Fluorescent Proteins by Two-Color Coincidence Detection and Nanophotonic Manipulation.The journal of physical chemistry. B · 2022Article
- High Macromolecular Crowding in Liposomes from Microfluidics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- SystematicACS synthetic biology · 2022Article
- The Effect of Lithium on the Budding YeastMicroorganisms · 2022Article
- Measurement of Macromolecular Crowding in Rhodobacter sphaeroides under Different Growth Conditions.mBio · 2022Article
- Förster resonance energy transfer-based kinase mutation phenotyping reveals an aberrant facilitation of CaFrontiers in molecular neuroscience · 2022Article
- A synthetic circuit for buffering gene dosage variation between individual mammalian cells.Nature communications · 2021Article
- Molecular Brightness Approach for FRET Analysis of Donor-Linker-Acceptor Constructs at the Single Molecule Level: A Concept.Frontiers in molecular biosciences · 2021Article
- Probing ion channel macromolecular interactions using fluorescence resonance energy transfer.Methods in enzymology · 2021Article
- Sensors for the quantification, localization and analysis of the dynamics of plant hormones.The Plant journal : for cell and molecular biology · 2021Review
- Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Förster resonance energy transfer (FRET)-based sensors are a valuable tool to quantify cell biology, yet it remains necessary to identify and prevent potential artifacts in order to exploit their full potential. We show here that artifacts arising from slow donor mCerulean3 maturation can be substantially diminished by constitutive expression in both prokaryotic and eukaryotic cells, which can also be achieved by incorporation of faster-maturing FRET donors. We developed an improved version of the donor mTurquoise2 that matures faster than the parent protein. Our analysis shows that using equal maturing fluorophores in FRET-based sensors or using constitutive low expression conditions helps to reduce maturation-induced artifacts, without the need of additional noise-inducing spectral corrections. In general, we show that monitoring and controlling the maturation of fluorescent proteins in living cells is important and should be addressed in in vivo applications of genetically encoded FRET sensors.
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