Evidence map›Paper›PMID 30168662›Full record

ArticleSmall (Weinheim an der Bergstrasse, Germany)2018

Standing Surface Acoustic Wave (SSAW)-Based Fluorescence-Activated Cell Sorter.

Liqiang Ren, Shujie Yang, Peiran Zhang, Zhiguo Qu, Zhangming Mao, Po-Hsun Huang, Yuchao Chen, Mengxi Wu, Lin Wang, Peng Li and 1 more

Abstract read
In one paragraph

Article in Small (Weinheim an der Bergstrasse, Germany), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed.

  1. Article
  2. Article
  3. Isolation Techniques of Micro/Nano-Scaled Species for Biomedical Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Review
  4. Article
  5. Article
  6. Review
  7. Review
  8. Review
  9. Article
  10. Article
  11. Review
  12. Article
  13. Article
  14. Review
  15. Review
  16. Article
  17. Article
  18. Acoustofluidic Droplet Sorter Based on Single Phase Focused Transducers.Small (Weinheim an der Bergstrasse, Germany) · 2021
    Article
  19. Article
  20. Acoustofluidics-Assisted Fluorescence-SERS Bimodal Biosensors.Small (Weinheim an der Bergstrasse, Germany) · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Liqiang RenDepartment of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA, 16802, USA.ORCID 0000-0003-0863-4609
Shujie YangDepartment of Mechanical Engineering and Materials Science, Duke University, Durham, NC, 27708, USA.
Peiran ZhangDepartment of Mechanical Engineering and Materials Science, Duke University, Durham, NC, 27708, USA.
Zhiguo QuKey Laboratory of Thermo-Fluid Science and Engineering, Ministry of Education, Xi'an Jiaotong University, Xi'an, Shaanxi, 710049, China.
Zhangming MaoAscent Bio-Nano Technologies, Inc., Research Triangle Park, NC, 27709, USA.
Po-Hsun HuangDepartment of Mechanical Engineering and Materials Science, Duke University, Durham, NC, 27708, USA.
Yuchao ChenDepartment of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA, 16802, USA.
Mengxi WuDepartment of Mechanical Engineering and Materials Science, Duke University, Durham, NC, 27708, USA.
Lin WangAscent Bio-Nano Technologies, Inc., Research Triangle Park, NC, 27709, USA.
Peng LiC. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV, 26506, USA.
Tony Jun HuangDepartment of Mechanical Engineering and Materials Science, Duke University, Durham, NC, 27708, USA.ORCID 0000-0003-1205-3313

Funding

Standing Surface Acoustic Wave Based Cell Sorters for Maintaining Cell IntegrityR01GM112048 · NIGMS · PENNSYLVANIA STATE UNIVERSITY, THE · PI HUANG, TONY JUN · 2014 to 2017
$1.1M
Validation of acoustic tweezers for single-cell analyses of purine metabolismR33EB019785 · NIBIB · PENNSYLVANIA STATE UNIVERSITY, THE · PI HUANG, TONY JUN · 2014 to 2016
$1.1M
NIBIB NIH HHS R33 EB019785NIGMS NIH HHS R01 GM112048
6 · The paper itself

Abstract

Microfluidic fluorescence-activated cell sorters (μFACS) have attracted considerable interest because of their ability to identify and separate cells in inexpensive and biosafe ways. Here a high-performance μFACS is presented by integrating a standing surface acoustic wave (SSAW)-based, 3D cell-focusing unit, an in-plane fluorescent detection unit, and an SSAW-based cell-deflection unit on a single chip. Without using sheath flow or precise flow rate control, the SSAW-based cell-focusing technique can focus cells into a single file at a designated position. The tight focusing of cells enables an in-plane-integrated optical detection system to accurately distinguish individual cells of interest. In the acoustic-based cell-deflection unit, a focused interdigital transducer design is utilized to deflect cells from the focused stream within a minimized area, resulting in a high-throughput sorting ability. Each unit is experimentally characterized, respectively, and the integrated SSAW-based FACS is used to sort mammalian cells (HeLa) at different throughputs. A sorting purity of greater than 90% is achieved at a throughput of 2500 events s

Indexed as

SoundFlow CytometryHeLa CellsHumansMicrofluidic Analytical Techniquesacoustofluidicsfluorescence-activated cell sorterssheathless focusingstanding surface acoustic waves

Identifiers

PMID30168662
PMCPMC6291339

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.