ArticleMolecular microbiology2018
SpoVID functions as a non-competitive hub that connects the modules for assembly of the inner and outer spore coat layers in Bacillus subtilis.
Article in Molecular microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Article
- Identification of SpoVID as a putative new substrate of the sporulation-specific protease YabG inFrontiers in microbiology · 2026Article
- To Sporulate or Not to Sporulate: Developmental Checkpoints MonitoringAnnual review of microbiology · 2025Review
- Real-time nanoscale investigation of spore coat assembly in Bacillus subtilis.Communications biology · 2025Article
- Embedding a ribonuclease in the spore crust couples gene expression to spore development in Bacillus subtilis.Nucleic acids research · 2025Article
- Bacterial spore surface nanoenvironment requires a AAA+ ATPase to promote MurG function.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Ultrastructure of macromolecular assemblies contributing to bacterial spore resistance revealed by in situ cryo-electron tomography.Nature communications · 2024Article
- A sporulation signature protease is required for assembly of the spore surface layers, germination and host colonization in Clostridioides difficile.PLoS pathogens · 2023Article
- Bacterial cell surface nanoenvironment requires a specialized chaperone to activate a peptidoglycan biosynthetic enzyme.bioRxiv : the preprint server for biology · 2023Article
- Bacterial developmental checkpoint that directly monitors cell surface morphogenesis.Developmental cell · 2022Article
- Molecular Physiological Characterization of a High Heat Resistant Spore FormingMicroorganisms · 2021Article
- A dynamic, ring-forming MucB / RseB-like protein influences spore shape in Bacillus subtilis.PLoS genetics · 2020Article
- Diversity and evolutionary dynamics of spore-coat proteins in spore-forming species of Bacillales.Microbial genomics · 2020Article
- From Root to Tips: Sporulation Evolution and Specialization in Bacillus subtilis and the Intestinal Pathogen Clostridioides difficile.Molecular biology and evolution · 2019Article
- Temporal and spatial regulation of protein cross-linking by the pre-assembled substrates of a Bacillus subtilis spore coat transglutaminase.PLoS genetics · 2019Article
- Contributions of crust proteins to spore surface properties in Bacillus subtilis.Molecular microbiology · 2019Article
- A LysM Domain Intervenes in Sequential Protein-Protein and Protein-Peptidoglycan Interactions Important for Spore Coat Assembly inJournal of bacteriology · 2019Article
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8 authors.
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Abstract
During sporulation in Bacillus subtilis, a group of mother cell-specific proteins guides the assembly of the coat, a multiprotein structure that protects the spore and influences many of its environmental interactions. SafA and CotE behave as party hubs, governing assembly of the inner and outer coat layers. Targeting of coat proteins to the developing spore is followed by encasement. Encasement by SafA and CotE requires E, a region of 11 amino acids in the encasement protein SpoVID, with which CotE interacts directly. Here, we identified two single alanine substitutions in E that prevent binding of SafA, but not of CotE, to SpoVID, and block encasement. The substitutions result in the accumulation of SafA, CotE and their dependent proteins at the mother cell proximal spore pole, phenocopying a spoVID null mutant and suggesting that mislocalized SafA acts as an attractor for the rest of the coat. The requirement for E in SafA binding is bypassed by a peptide with the sequence of E provided in trans. We suggest that E allows binding of SafA to a second region in SpoVID, enabling CotE to interact with E and SpoVID to function as a non-competitive hub during spore encasement.
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