ArticleScientific reports2018
A Versatile Sample Processing Workflow for Metagenomic Pathogen Detection.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers, 1 of them a synthesis that pooled it.
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Who cites it
70 citing papers in PubMed, 1 synthesis or guideline pooled it, 129 citations in OpenAlex.
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- An interlaboratory proficiency test using metagenomic sequencing as a diagnostic tool for the detection of RNA viruses in swine fecal material.Microbiology spectrum · 2024Article
- Lethal Borna disease virus 1 infections of humans and animals - in-depth molecular epidemiology and phylogeography.Nature communications · 2024Article
- Novel Pestiviruses Detected in Cattle Interfere with Bovine Viral Diarrhea Virus Diagnostics.Viruses · 2024Article
- A safe, effective and adaptable live-attenuated SARS-CoV-2 vaccine to reduce disease and transmission using one-to-stop genome modifications.Nature microbiology · 2024Article
- Genotyping of European Toxoplasma gondii strains by a new high-resolution next-generation sequencing-based method.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024Article
- Detection of Novel Poxvirus from Gray Seal (Halichoerus grypus), Germany.Emerging infectious diseases · 2023Article
- Field survey and molecular characterization of apicomplexan parasites in small mammals from military camps in Afghanistan.Parasitology research · 2023Article
- Bioinformatic Tools for NGS-Based Metagenomics to Improve the Clinical Diagnosis of Emerging, Re-Emerging and New Viruses.Viruses · 2023Review
- Characterisation of the Upper Respiratory Tract Virome of Feedlot Cattle and Its Association with Bovine Respiratory Disease.Viruses · 2023Article
- Mystery of fatal 'staggering disease' unravelled: novel rustrela virus causes severe meningoencephalomyelitis in domestic cats.Nature communications · 2023Article
- Archaeome structure and function of the intestinal tract in healthy and H1N1 infected swine.Frontiers in microbiology · 2023Article
- Continuous presence of genetically diverse rustrela virus lineages in yellow-necked field mouse reservoir populations in northeastern Germany.Virus evolution · 2023Article
- An advanced sequence clustering and designation workflow reveals the enzootic maintenance of a dominant West Nile virus subclade in Germany.Virus evolution · 2023Article
- Vaccine-induced Rabies in a Red Fox in Poland.Journal of veterinary research · 2022Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metagenomics is currently the only generic method for pathogen detection. Starting from RNA allows the assessment of the whole sample community including RNA viruses. Here we present our modular concerted protocol for sample processing for diagnostic metagenomics analysis of human, animal, and food samples. The workflow does not rely on dedicated amplification steps at any stage in the process and, in contrast to published methods, libraries prepared accordingly will yield only minute amounts of unclassifiable reads. We confirmed the performance of the approach using a spectrum of pathogen/matrix-combinations showing it has the potential to become a commonly usable analytical framework.
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