ArticleScientific reports2018
Self-replication of circular DNA by a self-encoded DNA polymerase through rolling-circle replication and recombination.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 74 citations in OpenAlex.
- A self-replicating artificial module-genome that generates bacterial chromosome replication system in vitro.Nucleic acids research · 2026Article
- PURE makes PURE: reconstitution of the PURE cell-free system from self-synthesized proteins.Nature communications · 2026Article
- DNA affects the phenotype of fuel-dependent coacervate droplets.Nature communications · 2026Article
- A synthetic cell with integrated DNA self-replication and lipid biosynthesis.Nature communications · 2026Article
- Autonomous biogenesis of all thirty proteins of the Escherichia coli translation machinery.Nature communications · 2025Article
- Reconstituting transcription-translation-coupled DNA replication within complex in vitro biological systems.Nature communications · 2025Article
- Orchestrating Self-Replication in Artificial Cells with Digital Microfluidics.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Simultaneous in vitro expression of minimal 21 transfer RNAs by tRNA array method.Nature communications · 2025Article
- Sustainable regeneration of 20 aminoacyl-tRNA synthetases in a reconstituted system toward self-synthesizing artificial systems.Science advances · 2025Article
- Self-growing protocell models in aqueous two-phase system induced by internal DNA replication reaction.Nature communications · 2025Article
- Artificial cells with all-aqueous droplet-in-droplet structures for spatially separated transcription and translation.Nature communications · 2025Article
- Darwinian Evolution of Self-Replicating DNA in a Synthetic Protocell.Nature communications · 2024Article
- H3.1K27me1 loss confers Arabidopsis resistance to Geminivirus by sequestering DNA repair proteins onto host genome.Nature communications · 2023Article
- C2CAplus: A One-Pot Isothermal Circle-to-Circle DNA Amplification System.ACS synthetic biology · 2023Article
- On biochemical constructors and synthetic cells.Interface focus · 2023Article
- Engineering Tissue-Scale Properties with Synthetic Cells: Forging One from Many.ACS synthetic biology · 2023Review
- Clonal Amplification-Enhanced Gene Expression in Synthetic Vesicles.ACS synthetic biology · 2023Article
- The Long Road to a Synthetic Self-Replicating Central Dogma.Biochemistry · 2023Review
- Progresses in Cell-Free In Vitro Evolution.Advances in biochemical engineering/biotechnology · 2023Review
- Phospholipid synthesis inside phospholipid membrane vesicles.Communications biology · 2022Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A major challenge in constructing artificial cells is the establishment of a recursive genome replication system coupled with gene expression from the genome itself. One of the simplest schemes of recursive DNA replication is the rolling-circle replication of a circular DNA coupled with recombination. In this study, we attempted to develop a replication system based on this scheme using self-encoded phi29 DNA polymerase and externally supplied Cre recombinase. We first identified that DNA polymerization is significantly inhibited by Cre recombinase. To overcome this problem, we performed in vitro evolution and obtained an evolved circular DNA that can replicate efficiently in the presence of the recombinase. We also showed evidence that during replication of the evolved DNA, the circular DNA was reproduced through recombination by Cre recombinase. These results demonstrate that the evolved circular DNA can reproduce itself through gene expression of a self-encoded polymerase. This study provides a step forward in developing a simple recursive DNA replication system for use in an artificial cell.
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