Evidence map›Paper›PMID 30166485›Full record

ReviewScience (New York, N.Y.)2018

Visualizing and discovering cellular structures with super-resolution microscopy.

Yaron M Sigal, Ruobo Zhou, Xiaowei Zhuang

Abstract readReview
In one paragraph

Review in Science (New York, N.Y.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 277 papers.

0numbers the graph read from it
0cells of the map it votes in
277citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

277 citing papers in PubMed.

  1. Article
  2. Review
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  10. Review
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  13. From Microscale to Nanoscale Shadow Electrochemiluminescence Microscopy.Angewandte Chemie (International ed. in English) · 2026
    Article
  14. Article
  15. Article
  16. Review
  17. The Fantastic Single-Molecule Techniques.Chemical & biomedical imaging · 2026
    Review
  18. Article
  19. Article
  20. Article

217 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Yaron M SigalHoward Hughes Medical Institute, Department of Chemistry and Chemical Biology, Department of Physics, Harvard University, Cambridge, MA 02138, USA.ORCID 0000-0001-9636-4164
Ruobo ZhouHoward Hughes Medical Institute, Department of Chemistry and Chemical Biology, Department of Physics, Harvard University, Cambridge, MA 02138, USA.ORCID 0000-0001-8628-0282
Xiaowei ZhuangHoward Hughes Medical Institute, Department of Chemistry and Chemical Biology, Department of Physics, Harvard University, Cambridge, MA 02138, USA. zhuang@chemistry.harvard.edu.ORCID 0000-0002-6034-7853

Funding

Generation of novel cell type specific mouse genetic toolsU19MH114830 · NIMH · ALLEN INSTITUTE · PI ZENG, HONGKUI · 2017 to 2021
$64.7M
Illuminating molecular mechanisms of cellular functions by single-molecule and super-resolution imagingR35GM122487 · NIGMS · HARVARD UNIVERSITY · PI ZHUANG, XIAOWEI · 2017 to 2021
$2.1M
Howard Hughes Medical InstituteNIGMS NIH HHS R35 GM122487NIMH NIH HHS U19 MH114830
6 · The paper itself

Abstract

Super-resolution microscopy has overcome a long-held resolution barrier-the diffraction limit-in light microscopy and enabled visualization of previously invisible molecular details in biological systems. Since their conception, super-resolution imaging methods have continually evolved and can now be used to image cellular structures in three dimensions, multiple colors, and living systems with nanometer-scale resolution. These methods have been applied to answer questions involving the organization, interaction, stoichiometry, and dynamics of individual molecular building blocks and their integration into functional machineries in cells and tissues. In this Review, we provide an overview of super-resolution methods, their state-of-the-art capabilities, and their constantly expanding applications to biology, with a focus on the latter. We will also describe the current technical challenges and future advances anticipated in super-resolution imaging.

Indexed as

AnimalsCellsHumansImaging, Three-DimensionalMicroscopy, FluorescenceMolecular ImagingNeuronsSynapses

Identifiers

PMID30166485
PMCPMC6535400

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.