Evidence map›Paper›PMID 30165406›Full record

ArticleJournal of radiation research2018

Determining survival fractions of Saccharomyces cerevisiae in response to ionizing radiation in liquid culture.

Xiaopeng Guo, Miaomiao Zhang, Yue Gao, Wenjian Li, Dong Lu

Open access · goldAbstract read
In one paragraph

Article in Journal of radiation research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.5field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Article
  3. Modulation of the nanoscale motion rate ofFrontiers in microbiology · 2023
    Article
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  5. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Xiaopeng GuoInstitute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China.
Miaomiao ZhangInstitute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China.
Yue GaoInstitute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China.
Wenjian LiInstitute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China.
Dong LuInstitute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China.
Chinese Academy of Sciences · CNInstitute of Modern Physics · CNUniversity of Chinese Academy of Sciences · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Saccharomyces cerevisiae survival fractions (SFs) in response to X-ray radiation were determined by two new methods: an OD600-based method and a 96-well method. For the OD600-based method, cells were exposed to various X-ray doses and inoculated into fresh medium: a lower biomass accumulated, indicating fewer surviving cells within the investigated dose range (0-100 Gy). For the 96-well method, diluent containing ~0-100 cells was equally divided into 96 droplets and respectively inoculated into 96 wells containing 200 μl of broth: fewer wells without S. cerevisiae clones indicated more surviving cells after 48 h of incubation. Corresponding quantitative systems were established. Both methods were sensitive and reliable. The OD600-based method is simple and fast, and the 96-well method simplifies the counting process. SF estimates by the OD600-based method were lower than those by 96-well methods owing to cell cycle arrest. In addition, comparisons of newly proposed and plate-counting methods indicated a higher rate of repair in S. cerevisiae in liquid culture than on agar.

Indexed as

Radiation, IonizingCell Culture TechniquesCell Cycle CheckpointsDose-Response Relationship, RadiationReproducibility of ResultsSaccharomyces cerevisiae

Identifiers

PMID30165406
PMCPMC6251422
OpenAlexW2888245179

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.