Evidence map›Paper›PMID 30165202›Full record

ArticleActa biomaterialia2018

Cartilage regeneration using arthroscopic flushing fluid-derived mesenchymal stem cells encapsulated in a one-step rapid cross-linked hydrogel.

Jun Li, Yongcan Huang, Jun Song, Xiaolin Li, Xintao Zhang, Zhiyu Zhou, Di Chen, Peter X Ma, Wenjing Peng, Wenxin Wang and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta biomaterialia, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.

0numbers the graph read from it
0cells of the map it votes in
37citing papers in PubMed
6.3field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

37 citing papers in PubMed, 79 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 3 countries.

Jun LiShenzhen Key Laboratory of Anti-Ageing and Regenerative Medicine, Department of Medical Cell Biology and Genetics, Guangdong Key Laboratory for Genome Stability & Disease Prevention, Shenzhen University Medical School, Shenzhen, Guangdong 518060, China; Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Guangdong, 518060, China.
Yongcan HuangShenzhen Engineering Laboratory of Orthopaedic Regenerative Technologies, Departments of Orthopaedics, Peking University Shenzhen Hospital, Shenzhen, Guangdong 510086, China.
Jun SongKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Guangdong, 518060, China.
Xiaolin LiShenzhen Key Laboratory of Anti-Ageing and Regenerative Medicine, Department of Medical Cell Biology and Genetics, Guangdong Key Laboratory for Genome Stability & Disease Prevention, Shenzhen University Medical School, Shenzhen, Guangdong 518060, China; The Charles Institute of Dermatology, School of Medicine and Medical Science, University College Dublin, Dublin, Ireland.
Xintao ZhangShenzhen Engineering Laboratory of Orthopaedic Regenerative Technologies, Departments of Orthopaedics, Peking University Shenzhen Hospital, Shenzhen, Guangdong 510086, China.
Zhiyu ZhouShenzhen Key Laboratory of Anti-Ageing and Regenerative Medicine, Department of Medical Cell Biology and Genetics, Guangdong Key Laboratory for Genome Stability & Disease Prevention, Shenzhen University Medical School, Shenzhen, Guangdong 518060, China.
Di ChenShenzhen Key Laboratory of Anti-Ageing and Regenerative Medicine, Department of Medical Cell Biology and Genetics, Guangdong Key Laboratory for Genome Stability & Disease Prevention, Shenzhen University Medical School, Shenzhen, Guangdong 518060, China; Department of Orthopedic Surgery, Rush University Medical Center, Chicago, IL 60612, USA.
Peter X MaSchools of Dentistry, Engineering and Medicine, University of Michigan, Ann Arbor, MI 48109-1078, USA.
Wenjing PengShenzhen Anhe Health Sciences Co. Ltd., Longhua District, Shenzhen, China.
Wenxin WangThe Charles Institute of Dermatology, School of Medicine and Medical Science, University College Dublin, Dublin, Ireland. Electronic address: wenxin.wang@ucd.ie.
Guangqian ZhouShenzhen Key Laboratory of Anti-Ageing and Regenerative Medicine, Department of Medical Cell Biology and Genetics, Guangdong Key Laboratory for Genome Stability & Disease Prevention, Shenzhen University Medical School, Shenzhen, Guangdong 518060, China. Electronic address: gqzhou@szu.edu.cn.
Shenzhen University · CNPeking University Shenzhen Hospital · CNUniversity College Dublin · IEUniversity of Michigan–Ann Arbor · US

Funding

The Mechanism through which TGF-Beta Maintains Chondrocytes in Proliferating StagR01AR054465 · NIAMS · UNIVERSITY OF ROCHESTER · PI CHEN, DI · 2007 to 2018
$2.7M
NIAMS NIH HHS R01 AR054465
6 · The paper itself

Abstract

Many attempts have been made to repair articular cartilage defects, including mesenchymal stem cell (MSC)-based tissue engineering strategies. Although this approach shows promise, optimizing MSC sources and their delivery is challenging. This study was designed to test the feasibility of using MSCs found in the human arthroscopic flushing fluid (AFF) for cartilage regeneration, by incorporating them into a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel. AFF-MSCs were isolated from the original intra-articular flushing fluid of 10 patients prior to arthroscopic procedures. The hydrogel was fabricated with hyper-branched polyPEGDA and thiolated hyaluronic acid (HA). In vitro assays demonstrated that AFF-MSCs possessed the typical MSC morphology and phenotype, and maintained chondrogenic differentiation properties when encapsulated within the hydrogel. The AFF-MSC/hydrogel composite could significantly repair full-thickness cartilage defects generated in a rat model after 8 weeks of implantation; smooth cartilage was formed with evidence of hyaline cartilage formation. These data suggest that human AFF-MSCs are a novel and abundant MSC source that have high therapeutic value for cartilage regeneration. STATEMENT OF SIGNIFICANCE: Many attempts have been made to repair the defects of articular cartilage, including mesenchymal stem cell (MSC)-based tissue engineering strategies. Optimizing MSC sources and their delivery approaches still remain clinically challenging. Recent studies determined that MSCs derived from synovium and synovial fluid exhibited superior chondrogenic potential. However, no feasible methods to harvest these human tissues and cells have been impeding them for clinical application. Hereby, we explored a simple and easy accessible approach to obtain a new stem cell source from arthroscopic flushing fluid (AFF-MSCs), which probably contains plenty of MSCs from synovium and synovial fluid. Further experiments demonstrated that encapsulation of these stem cells with one-step rapid cross-linked polyPEGDA/HA hydrogel held very encouraging potential for cartilage regeneration.

Indexed as

ArthroscopyRegenerationAgedAnimalsCartilage, ArticularCell DifferentiationCell ProliferationCell SeparationCells, ImmobilizedCell SurvivalCross-Linking ReagentsHumansHyaluronic AcidHydrogelsMaleMesenchymal Stem CellsCross-Linking ReagentsHyaluronic AcidHydrogelspoly(ethylene glycol)diacrylatePolyethylene GlycolsArthroscopic flushing fluidCartilage regenerationHydrogelMesenchymal stem cells

Identifiers

PMID30165202
PMCPMC6510982
OpenAlexW2889071254

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.