ArticleEuropean biophysics journal : EBJ2018
A simple cell-alignment protocol for sedimentation velocity analytical ultracentrifugation to complement mechanical and optical alignment procedures.
Article in European biophysics journal : EBJ, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Sedimentation velocity analytical ultracentrifugation (SV-AUC) for adeno-associated virus characterization: impact of cell alignment.European biophysics journal : EBJ · 2026Article
- Comparison of three AUC techniques for the determination of the loading status and capsid titer of AAVs.European biophysics journal : EBJ · 2023Article
- Analytical band centrifugation for the separation and quantification of empty and full AAV particles.Molecular therapy. Methods & clinical development · 2021Article
- Calibrating analytical ultracentrifuges.European biophysics journal : EBJ · 2021Article
- Probing the effect of aroma compounds on the hydrodynamic properties of mucin glycoproteins.European biophysics journal : EBJ · 2020Article
- Resuspending samples in analytical ultracentrifugation.Analytical biochemistry · 2020Article
- Efficient data acquisition with three-channel centerpieces in sedimentation velocity.Analytical biochemistry · 2019Article
- Analytical ultracentrifugation (AUC): a seminal tool offering multiple solutions.European biophysics journal : EBJ · 2018Article
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Authors and funding
4 authors.
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Abstract
In establishing the sources of data variability within sedimentation velocity analysis in the analytical ultracentrifuge and their relative importance, recent studies have demonstrated that alignment of the sample cells to the centre of rotation is the most significant contributing factor to overall variability, particularly for the characterisation of low levels of protein aggregation. Accurate mechanical and optical alignment tools have been recently designed. In this study, we (1) confirm the effect of misalignment observed by others on the estimated amounts of bovine serum albumin (BSA) monomer and dimer, and the sedimentation coefficient value for the BSA dimer; and (2) demonstrate the high performance of a mechanical alignment tool and the usefulness of a simple and complementary enhanced manual alignment protocol which should be useful for situations where these tools are not available.
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