Evidence map›Paper›PMID 30159002›Full record

ArticlePlant methods2018

Improving agroinfiltration-based transient gene expression in

Karlah Norkunas, Robert Harding, James Dale, Benjamin Dugdale

Open access · goldAbstract read
In one paragraph

Article in Plant methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 116 papers.

0numbers the graph read from it
0cells of the map it votes in
116citing papers in PubMed
16.6field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

116 citing papers in PubMed, 227 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Review
  8. Review
  9. Article
  10. Article
  11. Review
  12. Article
  13. Review
  14. Article
  15. Therapeutic Promise and Biotechnological Prospects ofInternational journal of molecular sciences · 2025
    Review
  16. Article
  17. Article
  18. Optimized expression of human interleukin-15 inBiotechnology reports (Amsterdam, Netherlands) · 2025
    Article
  19. OverexpressingPlants (Basel, Switzerland) · 2025
    Article
  20. Article

56 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Karlah NorkunasCentre for Tropical Crops and Biocommodities, Queensland University of Technology, Brisbane, QLD 4000 Australia.
Robert HardingCentre for Tropical Crops and Biocommodities, Queensland University of Technology, Brisbane, QLD 4000 Australia.
James DaleCentre for Tropical Crops and Biocommodities, Queensland University of Technology, Brisbane, QLD 4000 Australia.
Benjamin DugdaleCentre for Tropical Crops and Biocommodities, Queensland University of Technology, Brisbane, QLD 4000 Australia.ORCID 0000-0003-2281-4809
Queensland University of Technology · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAgroinfiltration is a simple and effective method of delivering transgenes into plant cells for the rapid production of recombinant proteins and has become the preferred transient expression platform to manufacture biologics in plants. Despite its popularity, few studies have sought to improve the efficiency of agroinfiltration to further increase protein yields. This study aimed to increase agroinfiltration-based transient gene expression in

resultsUsing the benchmark pEAQ-HT deconstructed virus vector system and the GUS reporter enzyme, physical, chemical, and molecular features were independently assessed for their ability to enhance

conclusionsIn this paper, different strategies were assessed and optimised with the aim of increasing plant-made protein capacities in

Indexed as

AgroinfiltrationHyperexpressionNicotiana benthamianapEAQ-HTTransient

Identifiers

PMID30159002
PMCPMC6109318
OpenAlexW2888279838

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.