ArticleFrontiers in neuroanatomy2018
Optimization of GFP Fluorescence Preservation by a Modified uDISCO Clearing Protocol.
Article in Frontiers in neuroanatomy, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 43 citations in OpenAlex.
- Pharmacological administration of FGF21 reverses obesity through a parabrachial-projecting neuron population in the hindbrain.Cell reports · 2026Article
- Current progress of labeling strategies in tissue clearing for large-scale biological visualization.Frontiers in cell and developmental biology · 2026Review
- In-depth 3D exploration of autosomal dominant polycystic kidney disease through light sheet fluorescence microscopy.Scientific reports · 2025Article
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- Deep imaging of LepRBone research · 2025Article
- 3D visualization of uterus and ovary: tissue clearing techniques and biomedical applications.Frontiers in bioengineering and biotechnology · 2025Review
- Efficient 3D imaging and pathological analysis of the human lymphoma tumor microenvironment using light-sheet immunofluorescence microscopy.Theranostics · 2024Article
- Long Preservation of AAV-Transduced Fluorescence by a Modified Organic Solvent-Based Clearing Method.International journal of molecular sciences · 2022Article
- Single-Step Fast Tissue Clearing of Thick Mouse Brain Tissue for Multi-Dimensional High-Resolution Imaging.International journal of molecular sciences · 2022Article
- Article
- Accelerated clearing and molecular labeling of biological tissues using magnetohydrodynamic force.Scientific reports · 2021Article
- FDISCO+: a clearing method for robust fluorescence preservation of cleared samples.Neurophotonics · 2021Article
- Can Developments in Tissue Optical Clearing Aid Super-Resolution Microscopy Imaging?International journal of molecular sciences · 2021Review
- A guidebook for DISCO tissue clearing.Molecular systems biology · 2021Review
- Urea-based amino sugar agent clears murine liver and preserves protein fluorescence and lipophilic dyes.BioTechniques · 2021Article
- Tissue clearing technique: Recent progress and biomedical applications.Journal of anatomy · 2021Review
- TISSUE CLEARING.Nature reviews. Methods primers · 2021Article
- Optical Tissue Clearing: Illuminating Brain Function and Dysfunction.Theranostics · 2021Review
- Tissue Optical Clearing for Biomedical Imaging: From In Vitro to In Vivo.Advances in experimental medicine and biology · 2021Article
- Biomedical Applications of Tissue Clearing and Three-Dimensional Imaging in Health and Disease.iScience · 2020Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tissue optical clearing techniques provide alternative approaches for imaging large-volume specimens. uDISCO, an organic-solvent-based method, stands out from the enormous array of available optical clearing methods by achieving whole-brain imaging with high transparency, size reduction and fluorescence preservation. In this study, we aimed to modify the uDISCO protocol to achieve better fluorescence preservation and to thereby further improve its optical imaging quality. First, we determined the optimal pH value for optimized uDISCO, termed "a-uDISCO" (alkaline pH-based uDISCO). Then, we compared fluorescence preservation between a-uDISCO and uDISCO. In addition, we validated the clearing performance of the optimized method according to several parameters, including tissue transparency, size changes, and the maintenance of cell morphology. Finally, we demonstrated that a-uDISCO enabled the high-quality brain-wide visualization of neuronal structures. This method potentially provides a better alternative for high-throughput imaging of samples with low-level fluorescence protein expression or for archiving and repetitive revisiting of rare samples.
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