Evidence map›Paper›PMID 30157937›Full record

ArticleStem cell research & therapy2018

Differentiation and transplantation of human induced pluripotent stem cell-derived otic epithelial progenitors in mouse cochlea.

Jianling Chen, Fanfan Hong, Cui Zhang, Liang Li, Cuicui Wang, Haosong Shi, Yong Fu, Jinfu Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed, 1 pooled it
3.6field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 1 synthesis or guideline pooled it, 59 citations in OpenAlex.

  1. Pooled it
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  6. Modern In Vitro Techniques for Modeling Hearing Loss.Bioengineering (Basel, Switzerland) · 2024
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  9. Article
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  13. New CRISPR Tools to Correct Pathogenic Mutations in Usher Syndrome.International journal of molecular sciences · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Jianling ChenInstitute of Cell and Development, College of Life Sciences, Zi-Jin-Gang Campus of Zhejiang University, Room 307, No.866, Yuhangtang Road, Hangzhou, 310058, Zhejiang, China.
Fanfan HongInstitute of Cell and Development, College of Life Sciences, Zi-Jin-Gang Campus of Zhejiang University, Room 307, No.866, Yuhangtang Road, Hangzhou, 310058, Zhejiang, China.
Cui ZhangInstitute of Cell and Development, College of Life Sciences, Zi-Jin-Gang Campus of Zhejiang University, Room 307, No.866, Yuhangtang Road, Hangzhou, 310058, Zhejiang, China.
Liang LiInstitute of Cell and Development, College of Life Sciences, Zi-Jin-Gang Campus of Zhejiang University, Room 307, No.866, Yuhangtang Road, Hangzhou, 310058, Zhejiang, China.
Cuicui WangInstitute of Cell and Development, College of Life Sciences, Zi-Jin-Gang Campus of Zhejiang University, Room 307, No.866, Yuhangtang Road, Hangzhou, 310058, Zhejiang, China.
Haosong ShiDepartment of Otorhinolaryngology, the Sixth People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
Yong FuDepartment of ENT, Head and Neck Surgery, the Children's Hospital, Zhejiang University School of Medicine, Zhejiang, China. 1307022@zju.edu.cn.
Jinfu WangInstitute of Cell and Development, College of Life Sciences, Zi-Jin-Gang Campus of Zhejiang University, Room 307, No.866, Yuhangtang Road, Hangzhou, 310058, Zhejiang, China. wjfu@zju.edu.cn.
Zhejiang University · CNChildren's Hospital of Zhejiang University · CNShanghai Sixth People's Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundInner ear hair cells as mechanoreceptors are extremely important for hearing. Defects in hair cells are a major cause of deafness. Induced pluripotent stem cells (iPSCs) are promising for regenerating inner ear hair cells and treating hearing loss. Here, we investigated migration, differentiation, and synaptic connections of transplanted otic epithelial progenitors (OEPs) derived from human iPSCs in mouse cochlea.

methodsHuman urinary cells isolated from a healthy donor were reprogramed to form iPSCs that were induced to differentiate into OEPs and hair cell-like cells. Immunocytochemistry, electrophysiological examination, and scanning electron microscopy were used to examine characteristics of induced hair cell-like cells. OEP-derived hair cell-like cells were cocultured with spiral ganglion neurons (SGNs), and the markers of synaptic connections were detected using immunocytochemistry and transmission electron microscope. In vivo, OEPs derived from iPSCs were transplanted into the cochlea of mice by injection through the round window. Migration, differentiation, and synaptic connections of transplanted cells were also examined by thin cochlear sectioning and immunohistochemistry.

resultsThe induced hair cell-like cells displayed typical morphological characteristics and electrophysiological properties specific to inner hair cells. In vitro, OEP-derived hair cell-like cells formed synaptic connections with SGNs in coculture. In vivo, some of the transplanted cells migrated to the site of the resident hair cells in the organ of Corti, differentiated into hair cell-like cells, and formed synaptic connections with native SGNs.

conclusionsWe conclude that the transplantation of OEPs is feasible for the regeneration of hair cells. These results present a substantial reference for a cell-based therapy for the loss of hair cells.

Indexed as

Gene Expression RegulationStem Cell TransplantationAdultAnimalsBiomarkersCell DifferentiationCell MovementCochleaCoculture TechniquesEpithelial CellsFemaleHair Cells, Auditory, InnerHumansInduced Pluripotent Stem CellsMiceMice, KnockoutBiomarkersSlc26a4 protein, mouseSulfate TransportersTranscription FactorsHair cell-like cellsInduced pluripotent stem cellsOtic epithelial progenitorsSensorineural hearing lossSynaptic connection

Identifiers

PMID30157937
PMCPMC6116394
OpenAlexW2889405054

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.